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Evaluation of The Efficiency of Various Commercial Products for The Bioremediation of Hydrocarbon Contaminated Soil

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Summary

Bioremediation has become an important method for the treatment of terrestrial oil spills and is often favoured over strictly physical-chemical methods. In this study, enzymatic analyses and signature lipid biomarkers were employed to evaluate the efficacy of selected bioremediation products on control and oil contaminated soil plots. It is envisioned that these biological indicators may be used as possible adjuncts to the strictly physical-chemical criteria most commonly employed. The application of the enzymatic and signature biomarker methods for product evaluation proved successful. The enzymatic assays provided a valuable insight into shifts in the functional diversity of the soil microbial communities resultant from the various treatments. Stimulation or inhibition of the microbial communities as a result of the various treatments was also demonstrated, particularly with regards to dehydrogenase activity. Phospholipid fatty acid profiles proved sufficiently sensitive to allow differentiation between products and resultant microbial communities that corresponded to satisfactory and unsatisfactory petroleum hydrocarbon removal.

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References

  • Alef, K. and Nannipieri, P. (eds.): 1995, Methods in Applied Soil Microbiology and Biochemistry, Academic Press, New York, p. 576.

    Google Scholar 

  • Aon, M.A., Cabello, M.N., Sarena, D.E., Colaneri, A.C., Franco, M.G., Burgos, J.L. and Cortassa, S.: 2001, ‘I. Spatio-temporal patterns of soil microbial and enzymatic activities in an agricultural soil,’ Appl. Soil Ecol. 18, 239–254.

    Google Scholar 

  • Atlas, R.M.: 1995, ‘Petroleum biodegradation and oil spill bioremediation,’ Mar. Pollut. Bull. 31, 178–182.

    CAS  Google Scholar 

  • Bandick, A.K. and Dick, R.P.: 1999, ‘Field management effects on soil enzyme activities,’ Soil Biol. Biochem. 31, 1471–1479.

    Article  CAS  Google Scholar 

  • Bento, F.M., Camargo, F.A.O., Okeke, B.C., and Frankenberger, W.T.: 2005, ‘Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation,’ Bioresour. Technol. 96, 1049–1055.

    Article  CAS  Google Scholar 

  • Bergmann, W. (ed.): 1992, Nutritional Disorders of Plants: Development, Visual and Analytical Diagnosis, Gustav Fischer, New York, p. 741.

    Google Scholar 

  • Dick, R.P., Breakwell, D.P. and Turco, R.F.: 1996, ‘Soil enzyme activities and biodiversity measurements as integrative microbiological indicators,’ in: J.W. Doran and A.J. Jones, (Eds.), Methods for Assessing Soil Quality, SSSA Special Publication 49, Soil Science Society of America, Madison, WI, pp. 247–271.

  • Dorn, P.B., Vipond, T.E., Salanitro, J.P. and Wisniewski, H.L.: 1998, ‘Assessment of the acute toxicity of crude oils in soils using earthworms, Microtox®, and plant,’ Chemosphere 37(5), 845–860.

    Article  CAS  Google Scholar 

  • Guckert, J.B., Ringelberg, B.D., White, D.C., Hanson, R.S. and Bratina, B.J.: 1991, ‘Membrane fatty acids as phenotypic markers in the polyphasic taxonomy of methylotrophs within the Proteobacteria,’ J. Gen. Microbiol. 137, 2631–2641.

    CAS  Google Scholar 

  • Guckert, J.B., Antworth, C.P., Nichols, P.D. and White, D.C.: 1985, ‘Phospholipid, ester-linked fatty acid profiles as reproducible assays for changes in prokaryotic community structure of estuarine sediments,’ FEMS Microbiol. Ecol. 31, 147–158.

    Article  CAS  Google Scholar 

  • Hicks, B.N., Caplan, J.A.: 1993, ‘Bioremediation: a natural solution,’ Pollut. Eng. 25, 30–33.

    CAS  Google Scholar 

  • Ibekwe, A.M. and Kennedy, A.C.: 1998, ‘Phospholipid fatty acid profiles and carbon utilization patterns for analysis of microbial community structure under field and greenhouse conditions,’ FEMS Microbiol. Ecol. 26, 151–163.

    CAS  Google Scholar 

  • Line, M.A., Garland, C.D. and Crowley, M.: 1996, ‘Evaluation of landfarm remediation of hydrocarbon-contaminated soil at the Inveresk railyard, Launceston, Australia,’ Waste Manag. 16, 567–570.

    Article  CAS  Google Scholar 

  • Maila, M.P. and Cloete, T.E.: 2005, ‘The use of biological activities to monitor the removal of fuel contaminants—perspective for monitoring hydrocarbon contamination: a review,’ Int. Biodeterior. Biodegradation. 55, 1–8.

    CAS  Google Scholar 

  • Pascual, J.A., Garcia, C., Hernandez, T., Moreno, J.L. and Ros, M.: 2000, ‘Soil microbial activity as a biomarker of degradation and remediation processes,’ Soil Biol. Biochem. 32, 1877–1883.

    Article  CAS  Google Scholar 

  • Ratledge, C., Wilkinson, S.G.: 1988, ‘An overview of microbial lipids,’ in C. Ratledge and S.G. Wilkinson (eds.), Microbial Lipids, Volume 1, Academic Press, London, pp. 3–22.

    Google Scholar 

  • Ritz, K., McHugh, M. and Harris, J.: 2003, ‘Biological diversity and function in soils: contemporary perspectives and implications in relation to the formulation of effective indicators,’ OECD Expert Meeting on Soil Erosion and Soil Biodiversity Indicators, Rome, March 2003, pp. 1–11.

  • Sabaté, J., Vias, M. and Solanas, A.M.: 2004, ‘Laboratory-scale bioremediation esperiments on hydrocarbon-contaminated soils,’ Int. Biodeterior. Biodegradation. 54, 19–25.

    Google Scholar 

  • Smith, C.A., Phiefer, C.B., Macnaughton, S.J., Peacock, A., Burkhalter, R.S., Kirkegaard, R. and White, D.C.: 2000, ‘Quantitative lipid biomarker detection of unculturable microbes and chlorine exposure in water distribution system biofilms,’ Water Res. 34, 2683–2688.

    Article  CAS  Google Scholar 

  • South Africa: 1991, The Minerals Act, No. 50 of 1991. Pretoria: Government Printer.

  • Steger, K., Jarvis, A., Smars, S. and Sundh, I.: 2003, ‘Comparison of signature lipid methods to determine microbial structure in compost,’ J. Microbiol. Methods. 55, 371–382.

    Article  CAS  Google Scholar 

  • Van Rensburg, L., De Sousa Correia, R.I., Booysen, J. and Ginster, M.: 1998, ‘Revegetation on a coal fine ach disposal site in South Africa,’ J. Environ. Qual. 27, 1479–1486.

    CAS  Google Scholar 

  • Vasudevan, N. and Rajaram, P.: 2001, ‘Bioremediation of oil sludge-contaminated soil,’ Environ. Int. 26, 409–411.

    Article  CAS  Google Scholar 

  • Voet, D. and Voet, J.G.: 1995, Biochemistry, 2nd edition, John Wiley & Sons, Inc., New York, p. 346.

    Google Scholar 

  • White, D.C., Stair, J.O. and Ringelberg, D.B.: 1996, ‘Quantitative comparisons of in situ microbial biodiversity by signature biomarker analysis,’ J. Ind. Microbiol. 17, 185–196.

    Article  CAS  Google Scholar 

  • White, D.C. and Ringelberg, D.B.: 1998, ‘Signature lipid biomarker analysis,’ in R.S. Burlage, R. Atlas, D. Stahl, G. Geesey, and G. Sayler, (ed.), Techniques in Microbial Ecology. Oxford University Press. New York, USA, pp. 255–272.

    Google Scholar 

  • Zelles, L.: 1999, ‘Fatty acid patterns of phospholipids and lipopolysaccharides in the characterization of microbial communities in soil: a review,’ Biol. Fertil. Soils 29, 111–129.

    Article  CAS  Google Scholar 

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Claassens, S., Van Rensburg, L., Riedel, K.J. et al. Evaluation of The Efficiency of Various Commercial Products for The Bioremediation of Hydrocarbon Contaminated Soil. Environmentalist 26, 51–62 (2006). https://doi.org/10.1007/s10669-006-5358-y

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