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Cadmium induces GAPDH- and- MDH mediated delayed cell aging and dysfunction in Candida tropicalis 3Aer

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Abstract

Eukaryotes employ various mechanisms to survive environmental stress conditions. Multicellular organisms eliminate permanently damaged cells by apoptosis, while unicellular eukaryotes like yeast react by decelerating cell aging. In the present study, transcriptomic and proteomic approaches were employed to elucidate the underlying mechanism of delayed apoptosis. Our findings suggest that Candida tropicalis 3Aer has a set of tightly controlled genes that are activated under Cd+2 exposition. Acute exposure to Cd+2 halts the cell cycle at the G2/M phase checkpoint and activates multiple cytoplasmic proteins that overcome effects of Cd+2-induced reactive oxygen species. Prolonged Cd+2 stress damages DNA and initiates GAPDH amyloid formation. This is the first report that Cd+2 challenge initiates dynamic redistribution of GAPDH and MDH and alters various metabolic pathways including the pentose phosphate pathway. In conclusion, the intracellular redistribution of GAPDH and MDH induced by prolonged cadmium stress modulates various cellular reactions, which facilitate delayed aging in the yeast cell.

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Acknowledgement

The authors express their gratitude to the Pakistan Science Foundation (PSF), Islamabad-Pakistan for support the present research work under project No. PSF/Res/ Envr (97).

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Authors and Affiliations

  1. University Institute of Medical Laboratory Technology (UIMLT), Faculty of Allied Health Sciences (FAHS), The University of Lahore, Lahore, Pakistan

    Zaman Khan

  2. Department of Microbiology, Government College University Faisalabad (GCUF), Jhang Road, Faisalabad, Pakistan

    Muhammad Atif Nisar & Saima Muzammil

  3. Department of Neurology, Clinical Dementia Center and DZNE, Georg-August University, University Medical Center Göttingen (UMG), Robert-Koch-Str. 40, 37075, Göttingen, Germany

    Saima Zafar & Inga Zerr

  4. Department of Microbiology and Molecular Genetics (MMG), University of the Punjab, New Campus, Lahore, 54590, Pakistan

    Abdul Rehman

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  1. Zaman Khan
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  2. Muhammad Atif Nisar
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Correspondence to Abdul Rehman.

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Khan, Z., Nisar, M.A., Muzammil, S. et al. Cadmium induces GAPDH- and- MDH mediated delayed cell aging and dysfunction in Candida tropicalis 3Aer. Environ Monit Assess 191, 490 (2019). https://doi.org/10.1007/s10661-019-7631-9

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