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Oxidative stress response in zebrafish (Danio rerio) gill experimentally exposed to subchronic microcystin-LR

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Abstract

The worldwide occurrence of cyanobacterial blooms makes it necessary to perform environmental risk assessment procedures to monitor the effects of microcytins on fish. Oxidative stress biomarkers are valuable tools in this regard. Considering that zebrafish (Danio rerio) is a common model species in fish toxicology and the zebrafish gill is potentially useful in screening waterborne pollutants, this study investigated the oxidative stress response in zebrafish gill exposed to subchronic microcystin-LR (MCLR) concentrations (2 or 20 μg/l) via measurement of toxin accumulation, protein phosphatase (PP) activity, and the antioxidant parameters (glutathione-S-transferase—GST; glutathione—GSH; superoxide dismutase—SOD; catalase—CAT; glutathione peroxide—GPx; glutathione reductase—GR), as well as levels of hydroxyl radical (OH) and lipid peroxidation (LPO). The results showed that after 30 days exposure, MCLR accumulated in zebrafish gill and MCLR exposure induced PP activity in gill. A linear inhibition of GST activity and GSH content was observed in the gills, revealing that they were involved in the first step of MCLR detoxification. The 2 μg/l MCLR treatment neglectably affected OH content and the antioxidant enzymes (SOD, CAT, GPx, and GR), however oxidative stress was induced under the 20 μg/l MCLR treatment in which an enhanced OH content and alterations of the antioxidant enzymes were observed in the treated gills, although both treatments exerted little effect on LPO level. The principal component analysis results indicated that the most sensitive biomarkers of MCLR exposure were GST and GSH in zebrafish gill. So, D. rerio could be regarded as a suitable bioindicator of MCLR exposure by measuring CAT, GR, GST, and GSH as biomarkers.

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Acknowledgments

This work was supported by open research founding for Integrated Marine Monitoring and Applied Technologies in Harmful Algal Blooms, and it was granted from the Key Laboratory of East China Sea Branch, State Oceanic Administration People’s Republic of China (MATHAB200901).

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  1. Xiamen Marine Environmental Monitoring Center, Xiamen, 361008, China

    Yao Chen, Song-Fu Zeng & Yu-Feng Cao

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  1. Yao Chen
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Chen, Y., Zeng, SF. & Cao, YF. Oxidative stress response in zebrafish (Danio rerio) gill experimentally exposed to subchronic microcystin-LR. Environ Monit Assess 184, 6775–6787 (2012). https://doi.org/10.1007/s10661-011-2457-0

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