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Bio-control of waterborne pathogens using Lactobacillus spp

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Abstract

Bacteria play a significant role in water contamination. Chemicals are mostly used for the treatment of bacteriologically contaminated water. The use of bacterial interactions is a new approach to limit the pathogens' growth. Detection of antimicrobial substances produced by lactic acid bacteria against the waterborne pathogens is the objective of this work. Microbiological and biochemical methods were used to identify lactic acid bacteria having an antimicrobial activity. Evaluation of antimicrobial activity with growth kinetic measurements was performed. Four isolates of lactic acid bacteria obtained from whey and curd were identified. The predominant species belonging to the Lactobacillus genera are: Lactobacillus rhamnosus, Lactobacillus sakei, Lactobacillus paracasei, and Lactobacillus paraplantarum. The present study revealed that the Lactobacillus consortium is able to inhibit Staphylococcus aureus's growth along with Escherichia coli and Vibrio species. In mixed culture, after 24 h, the Lactobacillus consortium reduces the growth of S. aureus by 2.03 log; moreover, the growth of the latter bacteria totally ceased after 72 h of incubation. The protein produced by the Lactobacillus consortium was responsible for arresting the growth of S. aureus.

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Acknowledgments

The authors express gratitude to the laboratory staff of EIRA Division, NEERI, Nagpur, for their valuable support during the entire study.

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Authors and Affiliations

  1. EIRA Division, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, 440020, India

    Ghyandeep L. Gaikwad & Prateksha Gupta

  2. National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, 440020, India

    Satish R. Wate

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  1. Ghyandeep L. Gaikwad
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  2. Prateksha Gupta
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Correspondence to Ghyandeep L. Gaikwad.

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Gaikwad, G.L., Gupta, P. & Wate, S.R. Bio-control of waterborne pathogens using Lactobacillus spp. Environ Monit Assess 184, 6627–6635 (2012). https://doi.org/10.1007/s10661-011-2447-2

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