Abstract
This study investigated the biological control effect and mechanism of Variovorax sp. R1 on cucumber gray mold. In vitro experiments showed that R1 disrupted the integrity of the cell membrane and cell wall of Botrytis cinerea (B. cinerea) and inhibited its spore germination rate and germ tube length. In vivo experiments showed that R1 could be attached to the cucumber wounds and significantly inhibit the incidence of cucumber. In addition, R1 could reduce the accumulation of ROS in cucumber by inducing the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) in cucumber. At the same time, the expression of the disease resistance gene (CsCHI, CsGLU, CsPAL, CsPR1) in cucumber increased after R1 treatment. Our study showed that R1 could control postharvest cucumber gray mold by directly inhibiting the growth of B. cinerea and inducing the activities of defense-related enzymes. Overall, R1 could effectively reduce gray mold of cucumber and has great potential for biological control.
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The datasets used or analysed during the current study are available from the corresponding author on reasonable request.
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This work was supported by National Key R & D Program of China (2021YFD2100100), the National Natural Science Foundation of China (32171836) and Natural Science Foundation of Liaoning Province (LJKZ0528, 2020-MZLH-37).
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Biying Yang, Zilong Li, and Shuhong Ye conceived and designed the experiments. Fengli Han and Yan Ding supervised the experimental process. Dan Chen and Min Zang contributed data analysis and mapping. Biying Yang, Zilong Li, and Min Zang wrote the manuscript. All authors read and approved the final manuscript.
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Yang, B., Li, Z., Ding, Y. et al. Effect of Ginkgo biloba endophytic bacterium Variovorax sp. R1 on the biological control of postharvest cucumber gray mold and related physiological mechanisms. Eur J Plant Pathol 167, 271–284 (2023). https://doi.org/10.1007/s10658-023-02706-y
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DOI: https://doi.org/10.1007/s10658-023-02706-y