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Occurrence and molecular variability of apple-associated Luteovirus isolates from apple in China based on coat protein genes

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Abstract

Apple-associated Luteovirus (AaLV) was recently found in apple trees in China. How commonly AaLV occurs in apple and how much genetic diversity is present among samples from various locations and apple cultivars are not reported but are investigated here. In this study, 162 samples from 13 locations, covering 13 apple cultivars of Malus pumila and one wild apple of Malus sieversii in China were collected, their coat protein (CP) genes were amplified by reverse transcription-polymerase chain reaction and the variability of the CP genes was analysed. The occurrence ratio of AaLV was 37.04% in apple trees in China, the AaLV isolates showed little variation on their CP genes, the geographic location or host cultivars had no consistent effect on the occurrence, variation and phylogenetic characters of AaLV. The results of this study will provide information on further study of the molecular characteristics and epidemiology of AaLV.

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Funding

This study was funded by the project of Research Start-up Funds for High-level Researchers in Inner Mongolia Agricultural University (NDYB2018-14), and Natural Science Foundation of Inner Mongolia Autonomous Region (2019MS03021).

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Authors and Affiliations

  1. College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Zhaowuda Road No. 306, Saihan, Inner Mongolia, Hohhot, 010018, China

    Zhengnan Li, Mingxiu Ju, Lei Zhang & Pingping Sun

  2. Citrus Research Institute, Southwest University, Tiansheng Road No. 2, Beifu, Chongqing, 400715, China

    Mengji Cao

Authors
  1. Zhengnan Li
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  2. Mingxiu Ju
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  3. Lei Zhang
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  4. Mengji Cao
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  5. Pingping Sun
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Corresponding authors

Correspondence to Mengji Cao or Pingping Sun.

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This research article is not submitted elsewhere for publication and this manuscript complies to the Ethical Rules applicable for this journal.

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This article does not contain any studies with human participants or animals performed by any of the authors. 

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The authors declare no conflict of interest and all the authors agreed to submit this manuscript in this current version.

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Li, Z., Ju, M., Zhang, L. et al. Occurrence and molecular variability of apple-associated Luteovirus isolates from apple in China based on coat protein genes. Eur J Plant Pathol 159, 421–426 (2021). https://doi.org/10.1007/s10658-020-02149-9

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  • DOI: https://doi.org/10.1007/s10658-020-02149-9

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