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Expression of lytic enzyme genes involved in antagonistic activity of Trichoderma viridescens during interaction with Sclerotinia sclerotiorum

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Abstract

This study was conducted to evaluate expression of 11 genes, endochitinase (chit33), endochitinase (chit37), endochitinase (chit42), N-acetyl-B-D-glucosaminidase (nag1), α-1,2-mannosidase (gh92), α-1,3-glucanase (a13gluc), β-1,6-glucanase (b16gluc), β-1,3-glucanase (b13gluc), aminopeptidase S9 (s9), trypsin-like (pra1) and serine protease (sprt) which may be involved in biological control of Sclerotinia sclerotiorum by Trichoderma viridescens. Total RNA was extracted from the mycelia where colonies of the pathogen and T. viridescens reached together on potato dextrose agar. As a control, a confrontation assay was conducted where T. viridescens was interacted with itself. The results of reverse transcriptase-PCR detected no band for s9 and sprt. However, expression of a13gluc, b16gluc, chit33 and chit37 was upregulated. No change in expressions was detected in chit42, b13gluc and pra1 compared with the control. The gh92 was upregulated only in one isolate (Tvig2). The expression of nag1 in both isolates of T. viridescens was clearly downregulated compared with the control. This study showed that upregulation of a13gluc, b16gluc, chit33 and chit37 in T. viridescens may play a role in biocontrol of S. sclerotiorum.

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Acknowledgments

This research was funded by Ton Duc Thang University, Ho Chi Minh City, Vietnam

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Authors and Affiliations

  1. Smart Agriculture Research and Application Team, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    Seyedmohammadreza Ojaghian

  2. Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    Seyedmohammadreza Ojaghian

  3. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China

    Ling Wang

  4. Ministry of Agriculture, Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China

    Liang Zhang

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  1. Seyedmohammadreza Ojaghian
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  2. Ling Wang
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Correspondence to Seyedmohammadreza Ojaghian.

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Ojaghian, S., Wang, L. & Zhang, L. Expression of lytic enzyme genes involved in antagonistic activity of Trichoderma viridescens during interaction with Sclerotinia sclerotiorum. Eur J Plant Pathol 157, 223–226 (2020). https://doi.org/10.1007/s10658-020-01992-0

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