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A new pin nematode, Paratylenchus sinensis n. sp. (Nematoda: Paratylenchinae) in the rhizosphere of white mulberry from Zhejiang Province, China

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Abstract

Pin nematode species are widely distributed and known to be associated with damage to a variety of shrubs, grasses, fruit and flowering trees. So far, no pin nematode species has been reported from Mulberry in China. During this study, a new Paratylenchus species recovered from the rhizosphere of mulberry tree, in Zhejiang Province is described and molecularly characterized. The species can be characterized by having swollen and slender females, four incisures in the lateral field, lip region conoid with anterior end flattened and without submedian lobes. The slender females have stylet 56.2 (50–62) μm long, excretory pore located at the level of nerve ring; vulval region with small advulval flap; tail conoid gradually tapers to form a finely pointed terminus. The swollen females comparatively larger than slender females, vulval lips enlarged, protruding and bulges out from the body contour, the tail is shorter than the slender female ending in a finely pointed terminus. The male is devoid of stylet, spicule 17.5 (16.8–18.2) μm long. Phylogenetic analysis based on partial 18S, ITS and D2-D3 expansion segments of the 28S rRNA gene sequences revealed that this new species grouped within paratylenchid species clade. Morphologically, this new species belongs to the Group 10 of Paratylenchus sensu lato (stylet >40 μm, 4 lateral lines and advulval flaps present).

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Notes

  1. The specific epithet is formed from the Latinised name of the country of origin.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 31772137). The authors thank Teacher Nianhang Rong from the Center of Electron Microscopy, Life Science Division, Zhejiang University, for providing assistance in the preparation of SEM.

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Authors and Affiliations

  1. Labrotory of Plant Nematology, Institute of Biotechnology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, 310058, Zhejiang, People’s Republic of China

    Munawar Maria, Wentao Miao & Jingwu Zheng

  2. Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario, Institute for Sustainable Agriculture (IAS), ceiA3, Avenida Menéndez Pidal s/n, 14004, Córdoba, Spain

    Pablo Castillo

  3. Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Hangzhou, 310058, People’s Republic of China

    Jingwu Zheng

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Maria, M., Miao, W., Castillo, P. et al. A new pin nematode, Paratylenchus sinensis n. sp. (Nematoda: Paratylenchinae) in the rhizosphere of white mulberry from Zhejiang Province, China. Eur J Plant Pathol 156, 1023–1039 (2020). https://doi.org/10.1007/s10658-020-01953-7

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