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Molecular and morphological characterization of the spiral nematode Helicotylenchus oleae Inserra, Vovlas & Golden, 1979 (Nematoda: Hoplolaimidae) in the Mediterranean Basin

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Abstract

The spiral nematode Helicotylenchus oleae is an ectoparasite of olive roots and is distributed in some countries in the Mediterranean Basin. In this study, we provided morphological and molecular characterisation of topotypes from southern Italy, as well as of several other populations from Crete (Greece) and Spain. Correct identification of plant-parasitic nematode species is essential to establish appropriate control strategies and for preventing their spread to other areas. Helicotylenchus oleae is reported for the first time in Greece (Crete). Integrative morphometric and molecular data for H. oleae populations using D2-D3 expansion segments of 28S rDNA, ITS-rDNA, and the cytochrome c oxidase subunit 1 (coxI), were in agreement with the original descriptions of the species, except for some minor differences, which may be a result of intraspecific variability. The phylogenetic relationships of this species with other representatives of Helicotylenchus spp. using D2-D3 expansion segments and the ITS-rRNA region was studied showing a low intraspecific diversity for H. oleae species. For the first time, coxI molecular data is obtained for the genus Helicotylenchus.

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Acknowledgements

Authors thank S. Santoro from Hortoservice, Nooicattaro, Bari, Italy, N. Trisciuzzi from CRSFA, Italy, J. Martín Barbarroja and G. León Ropero from IAS-CSIC for their help with sampling and the excellent technical assistance. This research was supported by grant P12-AGR 1486 from ‘Consejería de Economía, Innvovación y Ciencia’ from Junta de Andalucía, and Union Europea, Fondo Europeo de Desarrollo regional, ‘Una manera de hacer Europa’, grant 201740E042, "Análisis de diversidad molecular, barcoding, y relaciones filogenéticas de nematodos fitoparásitos en cultivos mediterráneos" from Spanish National Research Council (CSIC), grant KBBE 219262 ArimNET-ERANET FP7 2012-2015 Project PESTOLIVE ‘Contribution of olive history for the management of soilborne parasites in the Mediterranean basin’ from Hellenic Agricultural Organization-DEMETER and Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), and AGL-2012-37521 from ‘Ministerio de Economía y Competitividad’ of Spain.

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Authors and Affiliations

  1. Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario, ceiA3, Avenida Menéndez Pidal s/n, 14004, Córdoba, Spain

    Juan E. Palomares-Rius, Carolina Cantalapiedra-Navarrete, Antonio Archidona-Yuste & Pablo Castillo

  2. Consiglio Nazionale delle Ricerche (CNR), U.O.S. di Bari, Istituto per la Protezione Sostenibile delle Piante (IPSP), Via G. Amendola 122/D, 70126, Bari, Italy

    Nicola Vovlas

  3. Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, Institute of Olive Tree,Subtropical Crops and Viticulture,N.AG.RE.F., Hellenic Agricultural Organization-DEMETER, 32A Kastorias street, Mesa Katsabas, 71307, Heraklion, Crete, Greece

    Emmanuel A. Tzortzakakis

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  1. Juan E. Palomares-Rius
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  2. Carolina Cantalapiedra-Navarrete
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  4. Nicola Vovlas
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  5. Emmanuel A. Tzortzakakis
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  6. Pablo Castillo
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Correspondence to Juan E. Palomares-Rius.

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All the authors certify that 1) do not have any actual or potential conflict of interest, 2) the study described is original and has not been published previously, and is not under consideration for publication elsewhere, 3) all prevailing local, national and international regulations and conventions, and normal scientific ethical practices, have been respected. We also certify that all authors have reviewed the manuscript and approved the final version of manuscript before submission.

Research involving human participants and/or animals

No specific permits were required for the described fieldwork studies. Permission for sampling the crop orchards was granted by the landowner. The samples from wild plants were obtained in public areas, forests, and other natural areas studied and do not involve any species endangered or protected. The sites are not protected in any way.

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All the authors certify that the work carried out in this research followed the principles of ethical and professional conduct have been followed. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Palomares-Rius, J.E., Cantalapiedra-Navarrete, C., Archidona-Yuste, A. et al. Molecular and morphological characterization of the spiral nematode Helicotylenchus oleae Inserra, Vovlas & Golden, 1979 (Nematoda: Hoplolaimidae) in the Mediterranean Basin. Eur J Plant Pathol 150, 881–891 (2018). https://doi.org/10.1007/s10658-017-1330-6

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