Abstract
Impatiens necrotic spot virus (INSV) has been seen in many types of ornamental plants, including annuals and perennials. The virus is rapidly spread by the insect vector, the western flower thrips, and there is no cure for the disease. Due to its potential spread to other plants grown in the same area, it is very important to act quickly to remove and destroy affected plants. Therefore, a rapid diagnosis of INSV is crucial so that growers can determine if plants are diseased and act without delay. Loop-mediated isothermal amplification (LAMP) or reverse transcription LAMP (RT-LAMP) assay is a technique for amplifying DNA or RNA under a constant temperature, with high specificity, sensitivity, rapidity and efficiency. We developed a colorimetric immunocapture reverse transcription (IC-RT)-LAMP assay to detect INSV using RT-LAMP and a nucleic acid dye, GeneFinder™. We accordingly propose this assay as a highly reliable alternative viral recognition system for INSV detection.
References
Ahmadi, S., Almasi, M. A., Fatehi, F., Struik, P. C., & Moradi, A. (2012). Visual detection of Potato leafroll virus by one-step reverse transcription loop-mediated isothermal amplification of DNA with hydroxynaphthol blue dye. Phytopathology, 161, 120–124.
Almasi, M. (2015). Establishment and application of a reverse transcription loop-mediated isothermal amplification assay for detection of Grapevine fanleaf virus. Molecular Biology, 4, 149.
Almasi, M. A., Moradi, A., Nasiri, J., Karami, S., & Nasiri, M. (2012). Assessment of performance ability of three diagnostic methods for detection of Potato leafroll virus (PLRV) using different visualizing systems. Applied Biochemistry and Biotechnology, 168, 770–784.
Almasi, M. A., Erfanmanesh, M., Jafary, H., & Hosseinidehabadi, S. M. (2013). Visual detection of Potato leafroll virus by one-step reverse transcription loop-mediated isothermal amplification of DNA with the Genefinder™ dye. Journal of Virological Methods, 192, 51–54.
Almasi, M. A., Hosseyni-Dehabadi, S. M., & Aghapour-Ojaghkandi, M. (2014). Comparison and evaluation of three diagnostic methods for detection of Beet curly top virus in sugar beet using different visualizing systems. Applied Biochemistry and Biotechnology, 173, 1836–1848.
Almasi, M. A., Aghapour-ojaghkandi, M., Bagheri, K., Ghazvini, M., & Hosseyni-dehabadi, S. M. (2015). Comparison and evaluation of two diagnostic methods for detection of npt II and GUS genes in Nicotiana tabacum. Applied Biochemistry and Biotechnology, 175, 3599–3616.
Clark, M. F., & Adams, A. N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34, 475–483.
Daughtrey, M., Jones, R. K., Moyer, J. W., Daub, M. E., & Baker, J. R. (1997). Tospoviruses strike the greenhouse industry – INSV has become a major pathogen on flower crops. Plant Disease, 81, 1220–1230.
De Angelis, J. D., Sether, D. M., & Rossignol, P. A. (1994). Transmission of impatiens necrotic spot virus in peppermint by western flower thrips. Journal of Economic Entomology, 87, 197–201.
De Avila, A. C., De Haan, P., Kitajima, E. W., Kormelink, R., Resende, R. O., Goldbach, R. W., et al. (1992). Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from Impatiens sp. in the Netherlands. Journal of Phytopathology, 134, 133–151.
Dukes, J. P., King, D. P., & Alexandersen, S. (2006). Novels reverse transcription loop-mediated isothermal amplification for rapid detection of Foot-and-mouth disease virus. Archives of Virology, 151, 1093–1106.
Fukuta, S., Iida, T., Mizukami, Y., Ishida, A., Ueda, J., Kanbe, M., et al. (2003). Detection of Japanese yam mosaic virus by RT-LAMP. Archives of Virology, 148, 1713–1720.
Gracia, O., De Borbon, C. M., de Millan, N. G., & Cuesta, G. V. (1999). Occurrence of different tospoviruses in vegetable crops in Argentina. Journal of Phytopathology, 147, 223–227.
Hadersdorfer, J., Neumuller, M., Treutter, D., & Fischer, T. (2011). Fast and reliable detection of Plum pox virus in woody host plants using the blue LAMP protocol. Annals of Applied Biology, 159, 456–466.
Hill, J., Beriwal, S., Chandra, I., Paul, V. K., Kapil, A., Singh, T., et al. (2008). Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli. Journal of Clinical Microbiology, 46, 2800–2804.
Latham, L. J., & Jones, R. A. C. (1997). Occurrence of tomato spotted wilt tospovirus in native flora, weeds, and horticultural crops. Australian Journal of Agricultural Research, 48, 359–369.
Louro, D., & Kuo, C. G. (1997). Detection and identification of tomato spotted wilt virus and impatiens necrotic spot virus in Portugal. Acta Horticulturae, 431, 99–105.
Moradi, A., Nasiri, J., Abdollahi, H., & Almasi, M. (2012). Development and evaluation of a loop mediated isothermal amplification assay for detection of Erwinia amylovora based on chromosomal DNA. European Journal of Plant Pathology, 133, 609–620.
Moradi, A., Almasi, M. A., Jafary, H., & Mercado-Blanco, J. (2014). A novel and rapid loop-mediated isothermal amplification assay for the specific detection of Verticillium dahlia. Journal of Applied Microbiology, 116, 942–954.
Mori, Y., Kitao, M., Tomita, N., & Notomi, T. (2004). Real-time turbidimetry of LAMP reaction for quantifying template DNA. Journal of Biochemical and Biophysical Methods, 59, 145–157.
Nagamine, K., Hase, T., & Notomi, T. (2002). Accelerated reaction by loop mediated isothermal amplification using loop primers. Molecular and Cellular Probes, 16, 223–229.
Naidu, R. A., Deom, C. M., & Sherwood, J. L. (2001). First report of Frankliniella fusca as a vector of Impatiens necrotic spot tospovirus. Plant Disease, 85, 1211.
Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., et al. (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Research, 28, e63.
Ren, W. C., Wang, C. M., & Cai, Y. Y. (2009). Loop-mediated isothermal amplification for rapid detection of Acute viral necrobiotic virus in scallop chlamys farreri. Acta Virologica, 53, 161–167.
Roggero, P., Ogliara, P., Dellavalle, G., Lisa, V., Malavasi, F., Adam, G., et al. (1997). A general Tospovirus assay using monoclonal antibodies against tomato spotted wilt virus glycoproteins. Acta Horticulturae, 431, 167–175.
Sakurai, T., Inoue, T., & Tsuda, S. (2004). Distinct efficiencies of Impatiens necrotic spot virus transmission by five thrips vector species (Thysanoptera: Thripidae) of tospoviruses in Japan. Applied Entomology and Zoology, 39, 71–78.
Tomita, N., Mori, Y., Kanda, H., & Notomi, T. (2008). Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nature Protocols, 3, 877–882.
Tsai, S. M., Chan, K. W., Hsu, W. L., Chang, T. J., Wong, M. L., & Wang, C. Y. (2009). Development of a loop-mediated isothermal amplification for rapid detection of orf virus. Journal of Virological Methods, 157, 200–204.
Vicchi, V., & Bellardi, M. G. (1997). Impatiens necrotic spot tospovirus infection on lettuce in Italy. Informatore Fitopatologico, 47, 55–57.
Weekes, R. J., Mumford, R. A., Barker, I., Wood, K. R., & Kuo, C. G. (1997). Diagnosis of tospoviruses by reverse-transcription polymerase chain reaction. Acta Horticulturae, 431, 159–166.
Xia, J. Q., Sutula, C. L., & Marti, D. B. (1997). Development of a greenhouse test for tomato spotted wilt virus and impatiens necrotic spot virus. Acta Horticulturae, 431, 193–198.
Acknowledgments
We are grateful to Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran for financial support this study.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare no conflicts of interest.
Rights and permissions
About this article
Cite this article
Almasi, M.A., Almasi, G. Colorimetric immunocapture loop mediated isothermal amplification assay for detection of Impatiens necrotic spot virus (INSV) by GineFinder™ dye. Eur J Plant Pathol 150, 533–538 (2018). https://doi.org/10.1007/s10658-017-1294-6
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10658-017-1294-6