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Botryosphaeriaceae species associated with blueberry dieback and sources of primary inoculum in propagation nurseries in New Zealand

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Abstract

Sampling at blueberry farms found Botryosphaeriaceae fungi in five of seven farms sampled; overall incidence was 41.4%, with Neofusicoccum australe (79.0%), N. luteum (8.0%), N. ribis (8.0%) and N. parvum (5.0%). Sampling of nursery plants found infections in all four nurseries with 45% incidence in mainly asymptomatic plants, which were infected with N. australe (66.0%), N. parvum (31.5%) and N. ribis (2.5%). Asymptomatic propagation cuttings from one nursery were found to have external contamination of Botryosphaeriaceae DNA (90.0%) and internal infection (65.0%) by the four main species found in the blueberry farms and nurseries. For propagation media nested PCR showed that out of the 98 samples received, 43 samples were positive for the presence of Botryosphaeriaceae DNA (44.0%). Results from the SSCP indicated that N. australe, N. luteum, N. parvum/ N. ribis and Diplodia mutila were present in the propagation media received. Isolates of the four main species recovered from farms and nurseries were pathogenic on blueberry stems but pathogenicity differed significantly between species and isolates within a species, with N. ribis being the most pathogenic, then N. parvum, N. luteum and N. australe. Overall, the high rate of infection in nursery plants indicated that nurseries can be a major source of infection for blueberry farms. Since propagating cuttings are the likely sources of infection for nurseries, these should be targeted in the control strategies.

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References

  • Alves, A., Phillips, A. J. L., Henriques, I., & Correia, A. (2005). Evaluation of amplified ribosomal DNA restriction analysis (ARDRA) as a method for the identification of Botryosphaeria species. FEMS Microbiology Letters, 245, 221–229.

    Article  CAS  PubMed  Google Scholar 

  • Amponsah, N. T., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2009). First report of Neofusicoccum australe (Botryosphaeria australis), a cause of grapevine dieback in New Zealand. Australasian Plant Disease Notes, 4(1), 6–8.

    Article  CAS  Google Scholar 

  • Amponsah, N. T., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2011). Identification, potential inoculum sources and pathogenicity of botryosphaeriaceous species associated with grapevine dieback disease in New Zealand. European Journal of Plant Pathology, 131(3), 467–482.

    Article  Google Scholar 

  • Baskarathevan, J., Jaspers, M. V., Jones, E. E., & Ridgway, H. J. (2012). Incidence and distribution of botryosphaeriaceous species causing dieback and decline in New Zealand vineyards. European Journal of Plant Pathology, 132(4), 549–560.

    Article  Google Scholar 

  • Billones-Baaijens, R., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2013a). Virulence affected by assay parameters during grapevine pathogenicity studies with Botryosphaeriaceae nursery isolates. Plant Pathology, 62, 1214–1225.

    Article  Google Scholar 

  • Billones-Baaijens, R., Ridgway, H. J., Jones, E. E., Cruickshank, R. H., & Jaspers, M. V. (2013b). Prevalence and distribution of Botryosphaeriaceae species in New Zealand grapevine nurseries. European Journal of Plant Pathology, 135, 175–185.

    Article  Google Scholar 

  • Billones-Baaijens, R., Ridgway, H. J., Jones, E. E., & Jaspers, M. V. (2013c). Inoculum sources of Botryosphaeriaceae species in New Zealand grapevine nurseries. European Journal of Plant Pathology, 135, 159–174.

    Article  CAS  Google Scholar 

  • Blueberry Council, New Zealand. (2003). Blueberries 2002 statistical record. North American blueberry production. www.Blueberry.Org/publications/technical/tech%20brochure-sm.Pdf. 10.03.2013.

  • Creswell, T. C., & Milholland, R. D. (1988). Spore release and infection periods of Botryosphaeria dothidea on blueberry in North Carolina. Plant Disease, 72, 342–346.

    Article  Google Scholar 

  • Espinoza, J. G., Braceno, E. X., Chavez, E. R., Urbez-Torres, J. R., & Latorre, B. A. (2009). Neofusicoccum spp. associated with stem canker and dieback of blueberry in Chile. Plant Disease, 93(11), 1187–1194.

    Article  Google Scholar 

  • FreshFacts. (2007). http://www.hortresearch.co.nz/files/aboutus/factsandfigs/ff2007.pdf. Accessed 16 February 2013.

  • Milholland, R. D. (1971). Factors affecting sporulation and infection by the blueberry stem canker fungus, Botryosphaeria cortices. Phytopathology, 62, 137–139.

    Article  Google Scholar 

  • Milholland, R. D. (1995). Botryosphaeria stem blight. Pages 10–11 in. Compendium of Blueberry and Cranberry Diseases. Caruso F.L., and Ramsdell, D.C.(eds.), Compendium of blueberry and cranberry diseases, APS Press, St. Paul.

  • Pennycook, S. R., & Samuels, G. J. (1985). Botryosphaeria and Fusicoccum species associated with ripe fruit rot of Actinidia deliciosa (kiwifruit) in New Zealand. Mycotaxon, 24, 445–458.

    Google Scholar 

  • Ridgway, H. J., Amponsah, N. T., Brown, D. S., Jones, E. E., & Jaspers, M. V. (2011). Detection of botryosphaeriaceous species in environmental samples using a multi-species primer pair. Plant Pathology, 60, 1118–1127.

    Article  CAS  Google Scholar 

  • Sammonds, J., Billones, R., Rocchetti, M., Ridgway, H. J., Walter, M., & Jaspers, M. V. (2009). Survey of blueberry farms for Botryosphaeria dieback and crown rot pathogens. New Zealand Plant Protection, 62, 238–242.

    CAS  Google Scholar 

  • Smith, B. J. (2009). Botryosphaeria stem blight of southern blueberries: Cultivar susceptibility and effect of chemical treatments. Acta Horticulturae, 810, 385–394.

    Article  CAS  Google Scholar 

  • van Niekerk, J. M., Crous, P. W., Groenewald, J. Z., Fourie, P. H., & Halleen, F. (2004). DNA phylogeny, morphology and pathogenicity of Botryosphaeria species on grapevines. Mycologia, 96(4), 781–798.

    Article  PubMed  Google Scholar 

  • van Niekerk, J. M., Calitz, F. J., Hallen, F., & Fourie, P. H. (2010). Temporal spore dispersal patterns of grapevine trunk pathogens in South Africa. European of Journal Plant Pathology, 127, 375–390.

    Article  Google Scholar 

  • Wright, A. F., & Harmon, P. F. (2010). Identification of species in the Botryosphaeriaceae Family causing stem blight on southern highbush blueberry in Florida. Plant Disease, 94, 966–997.

    Article  Google Scholar 

  • Xu, C., Zhang, H., Zhou, Z., Hu, T., Wang, S., Wang, Y., & Cao, K. (2015). Identification and distribution of Botryosphaeriaceae species associated with blueberry stem blight in China. European Journal of Plant Pathology, 143(4), 737–752.

    Article  Google Scholar 

  • Yu, L., Rarisara, I., Xu, S. G., Wu, X., & Zhao, J. R. (2012). First report of stem blight of blueberry caused by Botryosphaeria dothidea in China. Plant Disease, 96(11), 1697.

    Article  Google Scholar 

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Acknowledgements

We thank Blueberries New Zealand and Lincoln University for funding this research and the blueberry growers who provided us with their valuable plant and propagation materials. Statistical advice was provided by Dr. Dean O’Connell.

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Authors and Affiliations

  1. Faculty of Agriculture and Life Sciences, Lincoln University, Box 85084, Lincoln, PO, New Zealand

    K. M. S. Tennakoon, Hayley J. Ridgway, Marlene V. Jaspers & E. Eirian Jones

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  1. K. M. S. Tennakoon
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  2. Hayley J. Ridgway
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  3. Marlene V. Jaspers
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  4. E. Eirian Jones
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Correspondence to E. Eirian Jones.

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Funding was provided by Lincoln University (Postgraduate research scholarship awarded to the first author) and Blueberries New Zealand. None of the authors declare a conflict of interest, with all authors consenting to publication.

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Tennakoon, K.M.S., Ridgway, H.J., Jaspers, M.V. et al. Botryosphaeriaceae species associated with blueberry dieback and sources of primary inoculum in propagation nurseries in New Zealand. Eur J Plant Pathol 150, 363–374 (2018). https://doi.org/10.1007/s10658-017-1283-9

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