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Disease suppressive effects of resistance-inducing agents against red rot of sugarcane

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Abstract

Red rot caused by Colletotrichum falcatum is one of the most devastating fungal diseases of sugarcane in many tropical countries. In this study, the efficacy of various resistance-inducing agents was evaluated against red rot at two growth phases of sugarcane, i.e., the germination and grand growth phases, for four consecutive cropping seasons (2011–12 to 2014–15) under field conditions. Inducer candidates of both synthetic origin (benzothiadiazole [BTH], salicylic acid [SA], potassium silicate [PSi]) and biotic origin (Colletotrichum falcatum [Cf] elicitor and Reynoutria sachalinensis extract) were evaluated for their efficacy on a red rot susceptible sugarcane cultivar CoC 671. Inducer concentrations that showed significant reduction of red rot lesion length in leaf bioassays without exhibiting direct antifungal activity were used in the field trials. Overall, results of the four field trials indicated that application of BTH (125 μM) and Cf elicitor (60 μg glucose equivalent/ml) were most efficient in reducing disease incidence during the germination and establishment phases against soil-borne inoculum and suppressed disease severity in pathogen challenged cane stalks during the grand growth phase. In addition, other treatments also had significant effects on germination and stalk disease severity. Quantification of C. falcatum biomass in resistance inducer-treated canes by quantitative PCR (qPCR) substantiated the disease suppressive effect of BTH and Cf elicitor. Whereas all resistance inducers significantly increased the cane weight, only BTH and PSi significantly increased the juice quantity. Other juice quality parameters were generally not affected by the inducers.

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References

  • Brodersen, P., Malinovsky, F. G., Hematy, K., Newman, M., & Mundy, J. (2005). The role of salicylic acid in the induction of cell death. Plant Physiology, 138, 1037–1045.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Clark, D. S. (1967). Comparison of pour and surface plate methods for determination of bacterial counts. Canadian Journal of Microbiology, 13, 1409–1412.

    Article  CAS  PubMed  Google Scholar 

  • Datnoff, L. E., Seebold, K. W., & Correa-V, F. J. (2001). The use of silicon for integrated disease management: reducing fungicide applications and enhancing host plant resistance. In L. E. Datnoff, G. H. Snyder (Eds.), Silicon in Agriculture (Vol. 8, pp. 171–183). Elsevier Science B.V.

  • Dean, J. V., Mohammed, L. A., & Fitzpatrick, T. (2005). The formation, vacuolar localization, and tonoplast transport of salicylic acid glucose conjugates in tobacco cell suspension cultures. Planta, 221, 287–296.

    Article  CAS  PubMed  Google Scholar 

  • Diguta, C. F., Rousseaux, S., Weidmann, S., Bretin, N., Vincent, B., Guilloux-Benatier, M., & Alexandre, H. (2010). Development of a qPCR assay for specific quantification of Botrytis cinerea on grapes. FEMS Microbiology Letters, 313, 81–87.

    Article  CAS  PubMed  Google Scholar 

  • Green, S. B., & Salkind, N. J. (2008). Using SPSS for Window and Macintosh: analyzing and understanding data. Pearson Prentice Hall (5th ed.), Upper Saddle River, NJ.

  • Hammerschmidt, R., & Kuc, J. (1995). Induced resistance to disease in plants. Dordrecht: Kluwer Academic Publishers.

    Book  Google Scholar 

  • Higgins, J. J. (2004). Introduction to modern nonparametric statistics. Pacific Grove: Duxbury Press.

    Google Scholar 

  • Honeycutt, R. J., Sobral, B. W. S., Keim, P., & Irvine, J. E. (1992). A rapid DNA extraction method for sugarcane and its relatives. Plant Molecular Biology Reporter, 10, 66–72.

    Article  CAS  Google Scholar 

  • Hsu, J. C. (1996). Multiple comparisons: theory and methods. London: Chapman & Hall.

    Book  Google Scholar 

  • Kloepper, W. J., Tuzun, S., & Kuc, A. J. (1992). Proposed definitions related to induced disease resistance. Biocontrol Science and Technology, 2, 349–351.

    Article  Google Scholar 

  • Kurabachew, H., & Wydra, K. (2014). Induction of systemic resistance and defense-related enzymes after elicitation of resistance by rhizobacteria and silicon application against Ralstonia solanacearum in tomato (Solanum lycopersicum). Crop Protection, 57, 1–7.

    Article  CAS  Google Scholar 

  • Lyon, G. (2007). Agents that can elicit induced resistance. In D. Walters, A. Newton, & G. Lyon (Eds.), Induced resistance for plant defense: a sustainable approach to crop protection (pp. 9–29). Oxford: Blackwell Publishing.

    Chapter  Google Scholar 

  • Malathi, P., Viswanathan, R., & Ramesh Sundar, A. (2016). Mechanized means of sett treatment: an effective way of delivering fungicides for the management of red rot in sugarcane. Sugar Technologie. doi:10.1007/s12355-016-0444-z.

    Google Scholar 

  • Muthiah, M., Ramadass, A., Amalraj, R. S., Palaniyandi, M., & Rasappa, V. (2013). Expression profiling of transcription factors (TFs) in sugarcane X Colletotrichum falcatum interaction. Journal of Plant Biochemistry and Biotechnology, 22, 286–294.

    Article  CAS  Google Scholar 

  • Pajerowska-Mukhtar, K. M., Emerine, D. K., & Mukhtar, M. S. (2013). Tell me more: roles of NPRs in plant immunity. Trends in Plant Science, 18, 402–411.

    Article  CAS  PubMed  Google Scholar 

  • Pieterse, C. M. J., & Van Loon, L. C. (2004). NPR1: the spider in the web of induced resistance signaling pathways. Current Opinion in Plant Biology, 7, 456–464.

    Article  CAS  PubMed  Google Scholar 

  • Ramesh Sundar, A., Velazhagan, R., & Vidhyasekaran, P. (2002). A glycoprotein elicitor isolated from Colletotrichum falcatum induces defense mechanisms in sugarcane leaves and suspension-cultured cells. Journal of Plant Diseases and Protection, 109, 601–611.

    Google Scholar 

  • Ramesh Sundar, A., Viswanathan, R., Malathi, P., & Padmanaban, P. (2006). Mechanism of resistance induced by plant activators against Colletotrichum falcatum in sugarcane. Archives of Phytopathology and Plant Protection, 39, 259–272.

    Article  Google Scholar 

  • Ramesh Sundar, A., Velazhahan, R., Nagarathinam, S., & Vidhyasekaran, P. (2008). Induction of pathogenesis-related proteins in sugarcane leaves and cell-cultures by a glycoprotein elicitor isolated from Colletotrichum falcatum. Biologia Plantarum, 52, 321–328.

    Article  Google Scholar 

  • Ramesh Sundar, A., Viswanathan, R., & Nagarathinam, S. (2009). Induction of systemic acquired resistance (SAR) using synthetic signal molecules against Colletotrichum falcatum in sugarcane. Sugar Technologie, 11, 274–281.

    Article  CAS  Google Scholar 

  • Ramesh Sundar, A., Selvaraj, N., Muthiah, M., Ramadass, A., Malathi, P., & Viswanathan, R. (2012). Induced resistance – a potential supplementary strategy for the management of red rot in sugarcane. In R. Viswanathan & A. Ramesh Sundar (Eds.), Sugarcane pathology - functional plant science and biotechnology (special issue) (pp. 63–72). United Kingdom: Global Science Books.

    Google Scholar 

  • Rivas-San Vicente, M., & Plasencia, J. (2011). Salicylic acid beyond defence: its role in plant growth and development. Journal of Experimental Botany, 62, 3321–3338.

    Article  CAS  PubMed  Google Scholar 

  • Šašek, V., Nováková, M., Dobrev, P. I., Valentová, O., & Burketová, L. (2012). β-aminobutyric acid protects Brassica napus plants from infection by Leptosphaeria maculans. Resistance induction or a direct antifungal effect? European Journal of Plant Pathology, 133, 279–289.

    Article  Google Scholar 

  • Sels, J., Mathys, J., De Coninck, B. M., Cammue, B. P., & De Bolle, M. F. (2008). Plant pathogenesis-related (PR) proteins: a focus on PR peptides. Plant Physiology and Biochemistry, 46, 941–950.

    Article  CAS  PubMed  Google Scholar 

  • Selvaraj, N., Ramadass, A., Amalraj, R. S., Palaniyandi, M., & Rasappa, V. (2014). Molecular profiling of systemic acquired resistance (SAR)-responsive transcripts in sugarcane challenged with Colletotrichum falcatum. Applied Biochemistry and Biotechnology, 174, 2839–2850.

    Article  CAS  PubMed  Google Scholar 

  • Shen, G., Xue, Q., Tang, M., Chen, Q., Wang, L., Duan, C., Xue, L., & Zhao, J. (2010). Inhibitory effects of potassium silicate on five soil-borne phytopathogenic fungi in vitro. Journal of Plant Diseases and Protection, 117, 180–184.

    Article  CAS  Google Scholar 

  • Sileshi, G. W. (2012). A critique of current trends in the statistical analysis of seed germination and viability data. Seed Science Research, 22, 145–159.

    Article  Google Scholar 

  • Singh, R. P., & Lal, S. (2000). Red rot. In Rott, P., Bailey, R. A., Comstock, J. C., Croft, B. J., Girard, J. C., & Saumtally, A. S (Eds.), A guide to sugarcane diseases (pp. 153–160). CIRAD, France: Quae.

  • Sundara, B. (1998). Sugarcane cultivation. New Delhi: Vikas publishers.

    Google Scholar 

  • Thode, H. C. (2002). Testing for normality. New York: Marcel Dekkers, Inc.

    Book  Google Scholar 

  • Van Dillewijn, C. (1952). Botany of sugarcane. Waltham: The Chronica Botanica Co.

    Google Scholar 

  • Van Loon, L. C. (1997). Induced resistance in plants and the role of pathogenesis-related proteins. European Journal of Plant Pathology, 103, 753–765.

    Article  Google Scholar 

  • Viswanathan, R. (2012). Sugarcane diseases and their management. Coimbatore: ICAR-Sugarcane Breeding Institute.

    Google Scholar 

  • Viswanathan, R., & Rao, G. P. (2011). Disease scenario and management of major sugarcane diseases in India. Sugar Technologie, 13, 336–353.

    Article  CAS  Google Scholar 

  • Viswanathan, R., Padmanaban, P., & Mohanraj, D. (1998). Comparison of three testing methods for the evaluation of red rot (Colletotrichum falcatum Went) in sugarcane. Indian Journal of Agricultural Science, 68, 226–230.

    Google Scholar 

  • Viswanathan, R., Malathi, P., & Padmanaban, P. (2003). Variation in sugarcane red rot pathogen Colletotrichum falcatum Went. In G. P. Rao, C. Manoharachari, D. J. Bhat, R. C. Rajak, & T. N. Lakhanpal (Eds.), Frontiers of fungal diversity in India (pp. 639–667). Lucknow: International Book Distributing Co.

    Google Scholar 

  • Viswanathan, R., Malathi, P., Ramesh Sundar, A., Aarthi, S., Premkumari, S. M., & Padmanaban, P. (2005). Differential induction of chitinases and thaumatin-like proteins in sugarcane in response to infection by Colletotrichum falcatum causing red rot disease. Journal of Plant Diseases and Protection, 112, 417–425.

    Google Scholar 

  • Viswanathan, R., Chinnaraja, C., Malathi, P., Gomathi, R., Rakkiyappan, P., Neelamathi, D., & Ravichandran, V. (2014). Impact of sugarcane yellow leaf virus (ScYLV) infection on physiological efficiency and growth parameters of sugarcane under tropical climatic conditions in India. Acta Physiologiae Plantarum, 36, 1805–1822.

    Article  CAS  Google Scholar 

  • Vlot, A. C., Dempsey, D. A., & Klessig, D. F. (2009). Salicylic acid, a multifaceted hormone to combat disease. Annual Review of Phytopathology, 47, 177–206.

    Article  CAS  PubMed  Google Scholar 

  • Wagih, M. E., Ala, A., & Musa, Y. (2004). Evaluation of sugarcane varieties for maturity earliness and selection for efficient sugar accumulation. Sugar Technologie, 6, 297–304.

    Article  CAS  Google Scholar 

  • Wallis, C. M., Wallingford, A. K., & Chen, J. (2013). Grapevine rootstock effects on scion sap phenolic levels, resistance to Xylella fastidiosa infection, and progression of Pierce’s disease. Frontiers in Plant Science, 4, 502.

    Article  PubMed  PubMed Central  Google Scholar 

  • Walters, D. R., & Fountaine, J. M. (2009). Practical application of induced resistance to plant diseases: an appraisal of effectiveness under field conditions. The Journal of Agricultural Science, 147, 523–535.

    Article  CAS  Google Scholar 

  • Walters, D., Walsh, D., Newton, A., & Lyon, G. (2005). Induced resistance for plant disease control: maximizing the efficacy of resistance elicitors. Phytopathology, 95, 1368–1373.

    Article  CAS  PubMed  Google Scholar 

  • Walters, D. R., Ratsep, J., & Havis, N. D. (2013). Controlling crop diseases using induced resistance: challenges for the future. Journal of Experimental Botany, 64, 1263–1280.

    Article  CAS  PubMed  Google Scholar 

  • Wobbrock, J. O., Findlater, L., Gergle, D., & Higgins, J. J. (2011). The aligned rank transform for nonparametric factorial analyses using only ANOVA procedures. In Proceedings of the SIGCHI Conference on Human Factors in Computing Systems, Vancouver, Canada, 143–146.

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Acknowledgements

The authors are grateful to the Director of the ICAR-Sugarcane Breeding Institute for providing facilities and continuous encouragement. The financial support received from the Indian Council of Agricultural Research and the Department of Science and Technology, New Delhi is gratefully acknowledged.

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Authors and Affiliations

  1. ICAR - Sugarcane Breeding Institute, Coimbatore, 641007, Tamil Nadu, India

    N. M. R. Ashwin, E. Leonard Barnabas, A. Ramesh Sundar, M. Muthumeena, P. Malathi & R. Viswanathan

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  1. N. M. R. Ashwin
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  2. E. Leonard Barnabas
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  4. M. Muthumeena
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Correspondence to A. Ramesh Sundar.

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Ashwin, N.M.R., Barnabas, E.L., Ramesh Sundar, A. et al. Disease suppressive effects of resistance-inducing agents against red rot of sugarcane. Eur J Plant Pathol 149, 285–297 (2017). https://doi.org/10.1007/s10658-017-1181-1

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