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ROS generation, oxidative burst and dynamic expression profiles of ROS-scavenging enzymes of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in response to Erwinia amylovora in pear (Pyrus communis L)

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Abstract

Here, the intensity and ratio of superoxide anion (O2 •), hydrogen peroxide (H2O2) and hydroxyl anion (OH•-) formation along the in vitro shootlets of four pear (Pyrus communis L.) rootstocks (i.e., Pyrodwarf, OH × F40, OH × F69 and OH × F333) were scrutinized under E. amylovora inoculation, over 144 hpi. Furthermore, following identifying the most tolerant and susceptible pear rootstocks (i.e., OH × F69 and OH × F40, respectively), the dynamic expression profiles of three ROS-scavenging enzymatic genes including superoxide dismutase (SOD), Catalase (CAT) and ascorbate peroxidase (APX) were elucidated in response to E. amylovora, over 96 hpi. The highest disease tolerance was observed in OH × F69, and OH × F333, Pyrodwarf and OH × F40 occupied the next descending positions, respectively. Furthermore, the O2 •- generation rates were almost similar in all the pears studied, though the accumulation of H2O2 and OH•- and intensities thereof were considerably distinctive and significantly followed up the levels of disease resistance. Comparing to the controls (0 hpi), in both susceptible and tolerant pear rootstocks, transcription activity of SOD, CAT, and APX genes were overall stimulated with relatively high abundance over 24, 48, 72 and 96 hpi, though some fluctuations were also recorded. Our ROS results, altogether, indicated that E. amylovora is capable enough to stimulate ROS formation in pear, though its progress is extremely dependent upon the susceptibility ratio of the plant. Lastly, the particular expression patterns and different response time of three genes designated that pear rootstocks differentially activates genes encoding antioxidant enzymes to mitigate the possible damage of ROS during E. amylovora invasion.

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Abbreviations

ANOVA:

Analysis of variance

APX:

peroxidase

AA:

Ascorbic acid

AUDPC:

Area under the disease progress curve

CAT:

Catalase

DMRT:

Duncans Multiple Range Test

E. amylovora :

Erwinia amylovora

EPSs:

Exopolysaccharides

HR:

Hypersensitive response

H2O2 :

Hydrogen peroxide

O2 :

Dioxygen

1O2 :

Singlet oxygen

OH•- :

Hydroxyl anion

O2 •- :

Superoxide anion

qRT-PCR:

Quantitative real-time PCR

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgments

This work was supported in part by special funds of Iranian Ministry of Agriculture, Seed and Plant Improvement Institute (SPII) for research on fire blight of pear and quince and a grant from Azad University, Varamin Branch of Tehran. The authors sincerely thank all the technical stuff of fruit tissue culture and biotechnology laboratory of SPII for their sincere supports.

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Authors and Affiliations

  1. Department of Plant Pathology, College of Agriculture, Varamin-Pishva Branch, Azad University, 33718, P.O.Box, Varamin, 57554, Iran

    Saeidreza Azarabadi

  2. Horticulture Research Department, Seed and Plant Improvement Institute, 4119, Karaj, 31585, Iran

    Hamid Abdollahi

  3. Department of Plant Pathology, College of Agriculture, Varamin-Pishva Branch, Azad University, 33718, P.O.Box, Varamin, 57554, Iran

    Mohamad Torabi

  4. Horticulture Research Department, Seed and Plant Improvement Institute, 31585, P.O.Box, Karaj, 4119, Iran

    Zeynab Salehi

  5. Division of Plant Molecular Genetics, Department of Agronomy and Plant Breeding, Agricultural & Natural Resources College, University of Tehran, Karaj, Iran

    Jaber Nasiri

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  1. Saeidreza Azarabadi
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  2. Hamid Abdollahi
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Correspondence to Hamid Abdollahi.

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Azarabadi, S., Abdollahi, H., Torabi, M. et al. ROS generation, oxidative burst and dynamic expression profiles of ROS-scavenging enzymes of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in response to Erwinia amylovora in pear (Pyrus communis L). Eur J Plant Pathol 147, 279–294 (2017). https://doi.org/10.1007/s10658-016-1000-0

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