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Description of a new needle nematode, Longidorus asiaticus n. sp. (Nematoda: Longidoridae), from the rhizosphere of crape myrtle (Lagerstroemia indica) bonsai trees imported into Italy from China

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Abstract

A new longidorid nematode, Longidorus asiaticus n. sp., is described and illustrated from a population extracted from soil associated with the movement of crape myrtle (Lagerstroemia indica) flowering bonsai trees imported from China into Italy. The new needle nematode is characterised by a small body size (2.74–3.52 mm), a bluntly-rounded lip region, ca 12 μm wide, continuous with body contour, amphidial fovea pocket-shape with posterior end rounded not bilobed, a moderately long and flexible odontostyle ca 85 μm long, stylet guiding ring located at ca 37 μm from anterior end, vulva almost equatorial (48–54 %), tail short, about 2/3 of its width, dorsally convex-conoid, with rounded terminus, with a c’ ratio ca 0.7, bearing two pairs of caudal pores and male absent. Integrative diagnosis was completed with molecular data obtained using D2-D3 expansion segments of 28S rDNA, ITS-rDNA, and partial 18S-rDNA. The phylogenetic relationships of this species with other Longidorus spp. using D2-D3 expansion segments, ITS and partial 18S-rDNA indicated that L. asiaticus n. sp. clustered together with L. hangzhouensis, Longidorus sp. JH-2014, and L. camelliae: all of them sharing a common Asiatic geographic origin.

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Notes

  1. The species epithet refers to Asia, the geographic origin of the species

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Acknowledgments

This research was partially supported by grant AGR-136 from ‘Consejería de Economía, Innvovación y Ciencia’ from Junta de Andalucía, and Union Europea, Fondo Europeo de Desarrollo regional, “Una manera de hacer Europa”. Authors thank J.E. Palomares-Rius and C. Cantalapiedra-Navarrete (IAS-CSIC) for critical reading of the manuscript before submission; and J. Martín Barbarroja and G. León Ropero from IAS-CSIC, and V. Radicci from IPSP-CNR for the excellent technical assistance. Authors thank all officers of Apulian Phytosanitary control service of Bari, Italy; and the commercial nursery for helping with detection and sanitation of this nematode.

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Authors and Affiliations

  1. Centro di Ricerca, Sperimentazione e Formazione in Agricoltura (CRSFA) “Basile Caramia”, Via Cisternino, 281, Locorotondo, BA, Italy

    Nicola Trisciuzzi

  2. Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario, ceiA3, 14004, Córdoba, Spain

    Antonio Archidona-Yuste & Pablo Castillo

  3. Istituto per la Protezione Sostenibile delle Piante (IPSP), Consiglio Nazionale delle Ricerche (CNR), U.O.S. di Bari, Via G. Amendola 122/D, 70126, Bari, Italy

    Alberto Troccoli, Elena Fanelli, Francesca De Luca & Nicola Vovlas

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  1. Nicola Trisciuzzi
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Correspondence to Pablo Castillo.

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Trisciuzzi, N., Archidona-Yuste, A., Troccoli, A. et al. Description of a new needle nematode, Longidorus asiaticus n. sp. (Nematoda: Longidoridae), from the rhizosphere of crape myrtle (Lagerstroemia indica) bonsai trees imported into Italy from China. Eur J Plant Pathol 143, 567–580 (2015). https://doi.org/10.1007/s10658-015-0710-z

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