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Preliminary studies on resistance phenotypes to Turnip mosaic virus in Brassica napus and B. carinata from different continents and effects of temperature on their expression

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Abstract

Preliminary studies were undertaken to establish the occurrence of Turnip mosaic virus (TuMV) resistance phenotypes in 99 Brassica napus and 32 B. carinata accessions, breeding lines and cultivars mostly from Africa, Australia, the Indian sub-continent or China. An isolate of TuMV pathotype 8 (WA-Ap1) was used in most inoculations. The influence of temperature on expression of resistance phenotypes was determined. Those identified were O (extreme resistance), RN (localised hypersensitivity), R (resistance to systemic movement without necrosis), +N (systemic infection with some necrosis), + (susceptibility), and RN/+ (systemic infection with necrosis limited to inoculated leaves). In the initial glasshouse evaluations with B. napus, 18 lines developed phenotype O, 42 RN, two + (both from Australia), and 30 segregated for O and RN, two for R and RN, one for O and R, two for O, R and RN, one for O, RN and +N, and one for RN and +N. Phenotype +N only occurred in two lines from India. In the initial glasshouse evaluations with B. carinata, 14 lines developed O, two RN or R, and one +; the 13 remaining lines segregated for phenotypes O and R (12), or RN and R (1). In B. carinata, phenotype RN only occurred in African lines, and phenotype + only in a line from Pakistan. The 18 B. napus and 14 B. carinata lines that developed phenotype O uniformly were re-evaluated at low (16 and/or 18 °C) and high (25 °C) temperatures in the glasshouse three or two times, respectively, and again under controlled environment room conditions at 16 and at 28 °C. While in most lines phenotype O was replaced by other phenotypes or segregated with other phenotypes at the higher temperatures, it occurred uniformly regardless of temperature in Chinese B. napus line (06-6-3777) and three African B. carinata lines (IP 117, ST 18 and ST 50). Graft inoculations confirmed extreme resistance in these four lines. Other promising lines which displayed phenotype O in >75 % of inoculated plants included for B. napus Chinese lines 06-p71-1 and 06-p74-4 and French line Cresor, and for B. carinata African lines Mbeya Green, ML-EM-1, ML-EM-7 and ML-EM-8, and Australian lines P 195923.3 and 30200533. Five promising lines segregated for phenotype RN, B. napus Ding 110, Hyola 42, Fan 028, ZY 007 and Qu 1104. Chinese B. napus line Ding 110 developed phenotype RN uniformly at high (25 and 28 °C) temperatures. Thus, in plants developing phenotypes RN, R and +, low temperature retarded virus multiplication in inoculated leaves to below the level at which it can be identified using ELISA. No clear phenotypic differences attributable to TuMV pathotype were found in tests in which isolates from pathotypes 1, 7 and 8 were used. This highlights the need for future evaluations for TuMV resistance in Brassica germplasm to be done at higher temperatures. This study also highlights the need to identify additional genes responsible for the different resistance and susceptibility reactions found, especially in B. carinata. The four lines that developed phenotype O uniformly at all temperatures and also withstood graft-inoculation will be particularly valuable for developing new TuMV-resistant cultivars of oilseed and forage Brassicas.

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Acknowledgments

The first author gratefully acknowledges the financial assistance of a Scholarship for International Research Fees (SIRF), operational funding support provided by the School of Plant Biology, The University of Western Australia and the Department of Agriculture and Food Western Australia, help with ELISA sample testing by Eva Gajda, and the provision of TuMV isolates by Brenda Coutts. Seeds of various Brassica lines used were provided by a previous Australian Centre for International Agricultural Research Project with India and China which was jointly funded with the Grain's Research and Development Corporation, or, for B. carinata lines, by the Asian Vegetable Research and Development Centre (AVRDC), Tanzania.

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  1. School of Plant Biology and Institute of Agriculture, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    Eviness P. Nyalugwe, Martin J. Barbetti & Roger A. C. Jones

  2. Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, WA, 6151, Australia

    Roger A. C. Jones

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  1. Eviness P. Nyalugwe
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Correspondence to Roger A. C. Jones.

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Nyalugwe, E.P., Barbetti, M.J. & Jones, R.A.C. Preliminary studies on resistance phenotypes to Turnip mosaic virus in Brassica napus and B. carinata from different continents and effects of temperature on their expression. Eur J Plant Pathol 139, 687–706 (2014). https://doi.org/10.1007/s10658-014-0423-8

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