Skip to main content
SpringerLink
Log in

Identification of new isolates of Turnip mosaic virus that cluster with less common viral strains

  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Turnip mosaic virus (TuMV) was found infecting cultivated brassicas and wild and cultivated ornamental Brassicaceae plants in different regions of Spain. Five new TuMV isolates, originating from different host plant species (Brassica cretica, Brassica juncea, Brassica napus, Eruca vesicaria subsp. sativa and Sisymbrium orientale), have been identified. The nucleotide sequences of the coat protein (CP) genes of the five isolates were determined. Phylogenetic analysis of the CP sequences showed that the five isolates grouped into two different clusters. The three isolates from the central region of Spain clustered with a previously reported Pisum sativum isolate from southeastern Spain, whereas the other two isolates from the eastern region clustered with two Italian and two Greek isolates. Both clusters were genetically distinct and belonged to the multi-lineage group OBR. The OBR group contains mainly TuMV isolates from hosts other than Brassica spp. and Raphanus sativus and mostly originating from Mediterranean countries. These new sequences provide further phylogenetic resolution of the OBR group. Although new TuMV isolates have been found in Spain, they were not associated with any serious disease outbreaks.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anonymous (2003) Anuario de Estadística Agroalimentaria

  • Brunt AA (1992) The general properties of potyviruses. In: Barnett OW (ed) Potyvirus taxonomy. Arch Virol Suppl 5: 3–16

  • JP Chen JP Chen MJ Adams (2002) ArticleTitleVariation between Turnip mosaic virus isolates in Zhejiang Province, China and evidence for recombination J Phytopathol 150 142–145 Occurrence Handle10.1046/j.1439-0434.2002.00731.x

    Article  Google Scholar 

  • F Garcia-Arenal A Fraile JM Malpica (2001) ArticleTitleVariability and genetic structure of plant virus populations Annu Rev Phytopathol 39 157–186 Occurrence Handle11701863 Occurrence Handle10.1146/annurev.phyto.39.1.157 Occurrence Handle1:CAS:528:DC%2BD3MXmvVygs70%3D

    Article  PubMed  CAS  Google Scholar 

  • A Gera DE Lesemann J Cohen A Franck S Levy R Salomon (1997) ArticleTitleThe natural occurrence of Turnip mosaic potyvirus in Allium ampeloprasum J Phytopathol 145 289–293

    Google Scholar 

  • D Gonsalves (1998) ArticleTitleControl of Papaya ringspot virus in papaya: a case study Annu Rev Phytopathol 36 415–437 Occurrence Handle15012507 Occurrence Handle10.1146/annurev.phyto.36.1.415 Occurrence Handle1:CAS:528:DyaK1cXmtlalt7k%3D

    Article  PubMed  CAS  Google Scholar 

  • FJ Jan SZ Pang C Fagoaga D Gonsalves (1999) ArticleTitle Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post-transcriptional gene silencing Transgenic Res 8 203–213 Occurrence Handle10478490 Occurrence Handle10.1023/A:1008915007271 Occurrence Handle1:CAS:528:DyaK1MXlvV2htb0%3D

    Article  PubMed  CAS  Google Scholar 

  • CE Jenner JA Walsh (1996) ArticleTitlePathotypic variation in turnip mosaic virus with special reference to European isolates Plant Pathol 45 848–856 Occurrence Handle10.1111/j.1365-3059.1996.tb02895.x

    Article  Google Scholar 

  • CE Jenner GJ Keane JE Jones JA Walsh (1999) ArticleTitleSerotypic variation in turnip mosaic virus Plant Pathol 48 101–108 Occurrence Handle10.1046/j.1365-3059.1999.00309.x

    Article  Google Scholar 

  • CE Jenner F Sanchez SB Nettleship GD Foster F Ponz JA Walsh (2000) ArticleTitleThe cylindrical inclusion gene of Turnip mosaic virus encodes a pathogenic determinant to the Brassica resistance gene TuRB01 Mol Plant-Microbe Interact 13 1102–1108 Occurrence Handle11043471 Occurrence Handle1:CAS:528:DC%2BD3cXntVarsrg%3D

    PubMed  CAS  Google Scholar 

  • CE Jenner K Tomimura K Ohshima SL Hughes JA Walsh (2002) ArticleTitleMutations in Turnip mosaic virus P3 and cylindrical inclusion proteins are separately required to overcome two Brassica napus resistance genes Virology 300 50–59 Occurrence Handle12202205 Occurrence Handle10.1006/viro.2002.1519 Occurrence Handle1:CAS:528:DC%2BD38Xmt12qsLo%3D

    Article  PubMed  CAS  Google Scholar 

  • S Kumar K Tamura M Nei (2004) ArticleTitleMEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment Brief Bioinform 5 150–163 Occurrence Handle15260895 Occurrence Handle10.1093/bib/5.2.150 Occurrence Handle1:CAS:528:DC%2BD2cXntFGqu7s%3D

    Article  PubMed  CAS  Google Scholar 

  • P Lehmann K Petrzik C Jenner A Greenland J Spak E Kozubek JA Walsh (1997) ArticleTitleNucleotide and amino acid variation in the coat protein coding region of turnip mosaic virus isolates and possible involvement in the interaction with the brassica resistance gene TuRB01 Physiol Mol Plant Pathol 51 195–208 Occurrence Handle10.1006/pmpp.1997.0116 Occurrence Handle1:CAS:528:DyaK1cXhs1aiur4%3D

    Article  CAS  Google Scholar 

  • B Moury C Morel E Johansen M Jacquemond (2002) ArticleTitleEvidence for diversifying selection in Potato virus Y and in the coat protein of other potyviruses J Gen Virol 83 2563–2573 Occurrence Handle12237440 Occurrence Handle1:CAS:528:DC%2BD38XnsFKnsrg%3D

    PubMed  CAS  Google Scholar 

  • L Nemchinov A Hadidi E Maiss M Cambra T Candresse V Damsteegt (1996) ArticleTitleSour cherry strain of plum pox potyvirus (PPV): molecular and serological evidence for a new subgroup of PPV strains Phytopathology 86 1215–1221 Occurrence Handle10.1094/Phyto-86-1215 Occurrence Handle1:CAS:528:DyaK28Xnt1ejsbs%3D

    Article  CAS  Google Scholar 

  • O Nicolas JF Laliberte (1992) ArticleTitleThe complete nucleotide sequence of turnip mosaic potyvirus RNA J Gen Virol 73 2785–2793 Occurrence Handle1431807 Occurrence Handle1:CAS:528:DyaK3sXkvVOks78%3D

    PubMed  CAS  Google Scholar 

  • K Ohshima Y Yamaguchi R Hirota T Hamamoto K Tomimura ZY Tan T Sano F Azuhata JA Walsh J Fletcher JS Chen A Gera A Gibbs (2002) ArticleTitleMolecular evolution of Turnip mosaic virus: evidence of host adaptation, genetic recombination and geographical spread J Gen Virol 83 1511–1521 Occurrence Handle12029167 Occurrence Handle1:CAS:528:DC%2BD38XktlGitb0%3D

    PubMed  CAS  Google Scholar 

  • F Sanchez X Wang CE Jenner JA Walsh F Ponz (2003) ArticleTitleStrains of Turnip mosaic potyvirus as defined by the molecular analysis of the coat protein gene of the virus Virus Res 94 33–43 Occurrence Handle12837555 Occurrence Handle10.1016/S0168-1702(03)00122-9 Occurrence Handle1:CAS:528:DC%2BD3sXkvF2iu7c%3D

    Article  PubMed  CAS  Google Scholar 

  • E Segundo G Martín-Bretones L Ruiz L Velasco D Janssen IM Cuadrado (2003) ArticleTitleFirst Report of Turnip mosaic virus in Pisum sativum in Spain Plant Dis 87 103 Occurrence Handle10.1094/PDIS.2003.87.1.103

    Article  Google Scholar 

  • VI Shattuck (1992) ArticleTitleThe biology, epidemiology, and control of turnip mosaic virus Plant Breed Rev 14 199–238

    Google Scholar 

  • L Stavolone D Alioto A Ragozzino J-F Laliberte (1998) ArticleTitleVariability among turnip mosaic potyvirus isolates Phytopathology 88 1200–1204 Occurrence Handle10.1094/PHYTO.1998.88.11.1200

    Article  Google Scholar 

  • Z Tan Y Wada J Chen K Ohshima (2004) ArticleTitleInter- and intralineage recombinants are common in natural populations of Turnip mosaic virus J Gen Virol 85 2683–2696 Occurrence Handle15302962 Occurrence Handle10.1099/vir.0.80124-0 Occurrence Handle1:CAS:528:DC%2BD2cXnsVegs7c%3D

    Article  PubMed  CAS  Google Scholar 

  • K Tomimura AJ Gibbs CE Jenner JA Walsh K Ohshima (2003) ArticleTitleThe phylogeny of Turnip mosaic virus; comparisons of 38 genomic sequences reveal a Eurasian origin and a recent ‘emergence’ in east Asia Mol Ecol 12 2099–2111 Occurrence Handle12859632 Occurrence Handle10.1046/j.1365-294X.2003.01881.x Occurrence Handle1:CAS:528:DC%2BD3sXmvFShtLY%3D

    Article  PubMed  CAS  Google Scholar 

  • K Tomimura J Spak N Katis CE Jenner JA Walsh AJ Gibbs K Ohshima (2004) ArticleTitleComparisons of the genetic structure of populations of Turnip mosaic virus in West and East Eurasia Virology 330 408–423 Occurrence Handle15567435 Occurrence Handle10.1016/j.virol.2004.09.040 Occurrence Handle1:CAS:528:DC%2BD2cXhtVaks7nP

    Article  PubMed  CAS  Google Scholar 

  • JA Tomlinson (1987) ArticleTitleEpidemiology and control of virus diseases of vegetables Ann Appl Biol 110 661–681 Occurrence Handle10.1111/j.1744-7348.1987.tb04187.x

    Article  Google Scholar 

  • JA Walsh CE Jenner (2002) ArticleTitle Turnip mosaic virus and the quest for durable resistance Mol Plant Pathol 3 289–300 Occurrence Handle10.1046/j.1364-3703.2002.00132.x Occurrence Handle1:CAS:528:DC%2BD38XotVSls7s%3D

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Madrid, Spain

    F. Sánchez, M. Rodríguez-Mateos, A. Touriño, J. Fresno & F. Ponz

  2. Departamento de Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Madrid, Spain

    C. Gómez-Campo

  3. Warwick HRI, University of Warwick, Wellesbourne, Warwick, U.K.

    C. E. Jenner & J. A. Walsh

Authors
  1. F. Sánchez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Rodríguez-Mateos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Touriño
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Fresno
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. Gómez-Campo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. E. Jenner
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. A. Walsh
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. F. Ponz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sánchez, F., Rodríguez-Mateos, M., Touriño, A. et al. Identification of new isolates of Turnip mosaic virus that cluster with less common viral strains. Arch Virol 152, 1061–1068 (2007). https://doi.org/10.1007/s00705-007-0943-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-007-0943-z

Keywords

Search

Navigation