Abstract
Regions within the mitochondrial gene encoding for the nicotinamide dehydrogenase subunit 4 (nad4) were characterized to evaluate the extent of genetic variation within and among Xiphinema diversicaudatum and Xiphinema simile populations. Four different sequence variants of nad4 were determined among eight populations of X. diversicaudatum and three variants among three populations of X. simile. Nucleotide variation was detected in 28 of 411 bp (2.43 to 4.87 %) in X. diversicaudatum and in three of 395 bp (0.25 to 0.76 %) in X. simile. This represents the first study based on the characterization of the nad4 gene for the analysis of population genetic of two Xiphinema species.
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Acknowledgements
The authors are thankful to Drs Lišková and Tiefenbrunner for providing nematodes. The work was supported by the Ministry of Agriculture of the Czech Republic, Project number MZe–0002700604.
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Kumari, S., Di Cesare, A. Nicotinamide dehydrogenase subunit 4 analysis of Xiphinema diversicaudatum and Xiphinema simile (Nematoda: Longidoridae). Eur J Plant Pathol 136, 803–810 (2013). https://doi.org/10.1007/s10658-013-0208-5
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DOI: https://doi.org/10.1007/s10658-013-0208-5