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Interaction of Collimonas strain IS343 with Rhizoctonia solani at low carbon availability in vitro and in soil

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Abstract

Collimonas sp. IS343, isolated from an organically-farmed arable soil and characterized as a broad-range oligotrophic bacterium, was shown to degrade chitin and to suppress R. solani mycelium growth under in vitro conditions at high and low carbon availabilities. In contrast to C. fungivorans Ter331, strain IS343 did not respond with an increase in growth rate to higher carbon levels in liquid medium, it reached higher cell numbers in carbon-poor media and it showed better survival in bulk soil. Therefore, it was concluded that strain IS343 cells are better adapted to circumstances of low carbon availability as present in bulk soils than strain Ter331 cells. Further, strain IS343 cells were more suppressive towards R. solani than strain Ter331 cells in vitro. When introduced into soil, strain IS343 cells delayed disease development caused by R. solani AG2-2IIIB in sugar beet plants. These results suggest that strain IS343 cells are able to tentatively suppress R. solani AG2-2IIIB mycelium growth in soil. Potential mechanisms behind the observed suppressive effects can be competition for available nutrients between strain IS343 cells and R. solani mycelium in soil or the production of chitinase as shown for this and other Collimonas species.

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Acknowledgments

This research was part of the Ecogenomics program which was sponsored by the Dutch National Genomics Initiative and the basic research program on sustainable agriculture (KB4) funded by the Dutch Ministry of Agriculture, Nature and Food Safety. We would like to thank Pieter Kastelein for his assistance with the plant-soil microcosm experiments.

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Authors and Affiliations

  1. Plant Research International BV, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Ilya V. Senechkin & Leonard S. van Overbeek

  2. Emerging Pathogens Institute and Plant Pathology Department, University of Florida, P.O. Box 110680, Gainesville, 32611, FL, USA

    Hong Ling Er & Ariena H. C. van Bruggen

  3. Farming Systems Ecology Group, Department of Plant Sciences, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Oscar de Vos

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  1. Ilya V. Senechkin
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  2. Leonard S. van Overbeek
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  3. Hong Ling Er
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  4. Oscar de Vos
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  5. Ariena H. C. van Bruggen
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Correspondence to Leonard S. van Overbeek.

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First and second authors contributed equally to the manuscript.

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Senechkin, I.V., van Overbeek, L.S., Er, H.L. et al. Interaction of Collimonas strain IS343 with Rhizoctonia solani at low carbon availability in vitro and in soil. Eur J Plant Pathol 136, 789–802 (2013). https://doi.org/10.1007/s10658-013-0207-6

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