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Effects of Pseudomonas fluorescens flagellin on physiological and biochemical characteristics in the suspension cells of Pinus thunbergii

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Abstract

In order to investigate the effects of flagellin secreted by bacteria associated with pine wood nematode (Bursaphelenchus xylophilus) on pine cells, suspension cells of Pinus thunbergii were treated with flagellin of Pseudomonas fluorescens GcM5-1A, and some physiological indices were determined. Superoxide anions radical (·O2 −) in treated suspension cells increased with prolongation of treatment time, with maximum content of 1,282 μmol g−1 FW at 30 h, but the content of H2O2 gradually decreased as compared with that in the control group. Maximum content of malondialdehyde (MDA) and free proline, which is 1.72 times and 1.49 times of that in the control, respectively, appeared when suspension cells were treated for 12 h, after that both of the contents decreased gradually. The activities of superoxide dismutase (SOD), peroxidase (POD) and catalases (CAT) also increased at the primary stage of treatment and then decreased compared with those of the control.

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Acknowledgment

This work was in part supported by National Natural Science Foundation of China (No: 31070575), Natural Science Foundation of Shandong Province, PR China (No: ZR2010CM009) and Basic Research Project of Qingdao, PR china (No: 12-1-4-2-(1)-jch).

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Authors and Affiliations

  1. Department of Biology, Qingdao University, 308 Ningxia Road, Qingdao, 266071, People’s Republic of China

    Zhongqiang Xu, Jie Yu, Limei Cui, Mei Li, Ronggui Li & Daosen Guo

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  1. Zhongqiang Xu
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  2. Jie Yu
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  3. Limei Cui
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  4. Mei Li
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  5. Ronggui Li
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  6. Daosen Guo
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Correspondence to Daosen Guo.

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Xu, Z., Yu, J., Cui, L. et al. Effects of Pseudomonas fluorescens flagellin on physiological and biochemical characteristics in the suspension cells of Pinus thunbergii . Eur J Plant Pathol 136, 729–736 (2013). https://doi.org/10.1007/s10658-013-0202-y

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  • DOI: https://doi.org/10.1007/s10658-013-0202-y

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