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Characterization of Longidorus helveticus (Nematoda: Longidoridae) from the Czech Republic

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Abstract

Longidorus helveticus was found at two out of 285 sampling sites for the first time in the Czech Republic. Females, males and juvenile stages were analyzed morphologically and morphometrically. The morphological identification of samples was verified by polymerase chain reaction using a species specific primer. Four markers of ribosomal DNA (18S, ITS1, ITS2, D2-D3 expansion segments of 28S rRNA) and two markers of mitochondrial DNA (cox1 and nad4) were sequenced and analyzed and compared with published gene sequences of other populations of L. helveticus. The partial mitochondrial cytochrome c-oxidase subunit 1 gene and partial nicotinamide dehydrogenase subunits 4 gene showed relatively high genetic variation within the species compared with ribosomal DNA markers.

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Acknowledgements

The work was supported by the Ministry of Agriculture of the Czech Republic, Project number MZe–0002700604 etapa 09.

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Authors and Affiliations

  1. Division of Plant Health, Crop Research Institute, Drnovská 507, Ruzyně, 16106, Prague 6, Czech Republic

    Shesh Kumari

  2. California Department of Food and Agriculture, Plant Pest Diagnostics Center, 3294 Meadowview Road, Sacramento, CA, 95832-1448, USA

    Sergei A. Subbotin

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  1. Shesh Kumari
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  2. Sergei A. Subbotin
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Correspondence to Shesh Kumari.

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Kumari, S., Subbotin, S.A. Characterization of Longidorus helveticus (Nematoda: Longidoridae) from the Czech Republic. Eur J Plant Pathol 133, 923–933 (2012). https://doi.org/10.1007/s10658-012-9959-7

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  • DOI: https://doi.org/10.1007/s10658-012-9959-7

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