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Morphological, pathological and genetic diversity of Colletotrichum species responsible for anthracnose in papaya (Carica papaya L)

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Abstract

Recently, anthracnose has become a major problem in papaya production and postharvest stages. The occurrence of both Colletotrichum gloeosporioides and Colletotrichum capsici has been demonstrated in this crop. The differential response of these pathogens to fungicides has highlighted the need to use rapid and accurate techniques to identify them. Thus, the objective of this study was to reveal the genetic diversity of Colletotrichum isolates in Mexican papaya fields. C. gloeosporioides-and C. capsici-specific primers were successfully used to detect the pathogens from different papaya parts. A combination of morphological characters, molecular techniques and pathogenicity tests were used to characterize 37 isolates from different localities of five papaya-producing states. Analyses of the 5.8-ITS region and arbitrarily primed-PCR revealed intraspecific groups; most of the isolates within these groups have the same geographical location and morphological characteristics. Knowledge of the genetic diversity of Colletotrichum spp. in Mexican papaya fields will facilitate the identification of the pathogen population in this crop in order to select the appropriate fungicide to control anthracnose, as well as to improve genetic resistance breeding programs.

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Abbreviations

AP-PCR:

Arbitrarily primed-polymerase chain reaction

d. a. i.:

Days after inoculation

ITS:

Internal transcribed spacers

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Acknowledgments

This research was supported by the Quintana Roo Produce Foundation through Grant 23-2009-1712. The authors thank Rodolfo Martin-Mex and Angel Nexticapan-Garcez for their valuable help in sample collection. Anuar Magaña-Alvarez and Gamaliel Itza-Kuk are thanked for their excellent technical assistance.

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Authors and Affiliations

  1. Laboratorio GeMBio, Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán, 97200, Mexico

    Claudia Torres-Calzada, Raul Tapia-Tussell & Daisy Perez-Brito

  2. Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán, 97200, Mexico

    Inocencio Higuera-Ciapara

Authors
  1. Claudia Torres-Calzada
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  2. Raul Tapia-Tussell
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  3. Inocencio Higuera-Ciapara
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  4. Daisy Perez-Brito
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Correspondence to Daisy Perez-Brito.

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Torres-Calzada, C., Tapia-Tussell, R., Higuera-Ciapara, I. et al. Morphological, pathological and genetic diversity of Colletotrichum species responsible for anthracnose in papaya (Carica papaya L). Eur J Plant Pathol 135, 67–79 (2013). https://doi.org/10.1007/s10658-012-0065-7

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