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PCR-Based Detection and Characterization of the Fungal Pathogens Colletotrichum gloeosporioides and Colletotrichum capsici Causing Anthracnose in Papaya (Carica papaya L.) in the Yucatan Peninsula

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Abstract

Colletotrichum gloeosporioides is the common causal agent of anthracnose in papaya (Carica papaya L.) fruits, and infection by this fungal pathogen results in severe post-harvest losses. In the Yucatán peninsula (Mexico) a different Colletotrichum species was isolated from papaya fruits with atypical anthracnose lesions. The DNAs from a variety of Colletotrichum isolates producing typical and atypical lesions, respectively, were amplified by PCR with C.gloeosporioides-specific primers. All isolates from typical anthracnose lesions yielded a 450 bp PCR product, but DNAs from isolates with atypical lesions failed to produce an amplification product. For further characterization, the rDNA 5.8S-ITS region was amplified by PCR and processed for sequencing and RFLP analysis, respectively, to verify the identity of the papaya anthracnose pathogens. The results revealed unequivocally the existence of two Colletotrichum species causing anthracnose lesions on papaya fruits: C. gloeosporioides and C. capsici. PCR-RFLP using the restriction endonuclease MspI reliably reproduced restriction patterns specific for C. capsici or C. gloeosporioides. The generation of RFLP patterns by MspI (or AluI or RsaI) is a rapid, accurate, and unequivocal method for the detection and differentiation of these two Colletotrichum species.

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Acknowledgements

The authors are grateful to Dr. Jairo Alejo for providing some of the samples of papaya fruits with anthracnose used in this study; and especially to Prof. Dr. Wolfgang Rohde, from Max Planck Institute for Plant Breeding Research, for his complete review of this manuscript and his time and effort in providing comments.

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Authors and Affiliations

  1. Laboratorio GeMBio, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, Merida, Yucatan, 97200, Mexico

    Raul Tapia-Tussell, Andres Quijano-Ramayo, Alberto Cortes-Velazquez & Daisy Perez-Brito

  2. Instituto de Biología, Universidad Nacional Autónoma de México, 3er Circuito S/N, Ciudad Universitaria, Del. Coyoacán, Mexico, DF, 04510, Mexico

    Patricia Lappe

  3. Centro de Investigación Científica de Yucatán, Calle 43 # 130, Col. Chuburná de Hidalgo, Merida, Yucatan, 97200, Mexico

    Alfonso Larque-Saavedra

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  1. Raul Tapia-Tussell
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  2. Andres Quijano-Ramayo
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  3. Alberto Cortes-Velazquez
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  4. Patricia Lappe
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  6. Daisy Perez-Brito
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Correspondence to Daisy Perez-Brito.

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Tapia-Tussell, R., Quijano-Ramayo, A., Cortes-Velazquez, A. et al. PCR-Based Detection and Characterization of the Fungal Pathogens Colletotrichum gloeosporioides and Colletotrichum capsici Causing Anthracnose in Papaya (Carica papaya L.) in the Yucatan Peninsula. Mol Biotechnol 40, 293–298 (2008). https://doi.org/10.1007/s12033-008-9093-0

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  • DOI: https://doi.org/10.1007/s12033-008-9093-0

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