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The influence of Aphanomyces cochlioides on selected physiological processes in sugar beet leaves and yield parameters

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Abstract

Alterations in some physiological processes in source leaves of sugar beet—such as chlorophyll and carbohydrate concentrations, stomatal conductance, rate of net photosynthesis and transpiration, and activity of the photosynthetic apparatus during root interaction with Aphanomyces cochlioides, were investigated. The influence of time of infection on plant health, yield quality and quantity was also examined. Plants were infected at different times of their growth period: on the sowing day and 4 or 8 weeks after sowing. A variation treatment, with non-pelleted seeds infected on the sowing day, was also analyzed. The experiment showed that development of disease symptoms depends on the time of infection and seed protection. A significant root yield decrease was observed in case of late infection, as compared to the yield of plants infected on the sowing day. The fresh weight of leaves was significantly increased where there was late infection. The infected plants showed a lower content of K+, Na+ and α-amino-N than did the controls. Infection by A. cochlioides induced chlorophyll degradation mostly in older leaves with the occurrence of natural senescence processes. Chlorophyll fluorescence parameters indicated that the photosynthetic apparatus of younger leaves was more sensitive to pathogen infection, when compared to older ones. The photochemical efficiency of photosystem II was reduced in young leaves mainly due to disturbance of the water-splitting system. In plants grown from non-pelleted seeds a strong impairment of PSII was observed only in those leaves which developed during early pathogen infection. In young leaves of plants infected in the fourth week after sowing, inhibition of the rate of net photosynthesis was correlated with the increase in intercellular CO2 concentration, indicating some disturbance in the carbon assimilation phase. In mature leaves of late infected plants the reduction of photosynthesis net rate was associated with a decrease of stomatal conductance and an increase of diffusion resistance to CO2 and H2O, which was also the cause of the transpiration rate inhibition. When the leaves developed during early infection, an increase of specific leaf weight and accumulation of carbohydrates was observed. In mature leaves of non-protected plants infected on the sowing day, the recovery of all physiological processes was observed together with a diminution of disease symptoms.

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Acknowledgements

The research was financially supported by KWS Polska and Südzucker Polska.

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Authors and Affiliations

  1. Department of Plant Physiology, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, SGGW, ul. Nowoursynowska 159, 02-776, Warsaw, Poland

    Danuta Chołuj

  2. Faculty of Natural and Technical Sciences, Opole University, ul. Kard. B. Kominka 6a, 45-035, Opole, Poland

    Ewa B. Moliszewska

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  1. Danuta Chołuj
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  2. Ewa B. Moliszewska
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Correspondence to Danuta Chołuj.

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Chołuj, D., Moliszewska, E.B. The influence of Aphanomyces cochlioides on selected physiological processes in sugar beet leaves and yield parameters. Eur J Plant Pathol 132, 59–70 (2012). https://doi.org/10.1007/s10658-011-9848-5

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  • DOI: https://doi.org/10.1007/s10658-011-9848-5

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