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Aggressiveness and mycotoxin production of eight isolates each of Fusarium graminearum and Fusarium verticillioides for ear rot on susceptible and resistant early maize inbred lines

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Abstract

Fusarium graminearum and F. verticillioides are among the most important pathogens causing ear rot of maize in Central Europe. Our objectives were to (1) compare eight isolates of each species on two susceptible inbred lines for their variation in ear rot rating and mycotoxin production across 3 years, and (2) analyse two susceptible and three resistant inbred lines for potential isolate x line interactions across 2 years by silk-channel inoculation. Ear rot rating, zearalenone (ZEA) and deoxynivalenol (DON) concentrations were evaluated for all F. graminearum isolates. In addition, nivalenol (NIV) concentrations were analysed for two NIV producers. Fumonisin (FUM) concentrations were measured for all F. verticillioides isolates. Mean ear rot severity was highest for DON producers of F. graminearum (62.9% of the ear covered by mycelium), followed by NIV producers of the same species (24.2%) and lowest for F. verticillioides isolates (9.8%). For the latter species, ear rot severities differed highly among years (2006: 24%, 2007: 3%, 2008: 7%). Mycotoxin concentrations among isolates showed a broad range (DON: 100–284 mg kg−1, NIV: 15–38 mg kg−1, ZEA: 1.1–49.5 mg kg−1, FUM: 14.5–57.5 mg kg−1). Genotypic variances were significant for isolates and inbred lines in all traits and for both species. Isolate x line interactions were significant only for ear rot rating (P < 0.01) and DON concentration (P < 0.05) of the F. graminearum isolates, but no rank reversals occurred. Most isolates were capable of differentiating the susceptible from the resistant lines for ear rot severity. For resistance screening, a sufficiently aggressive isolate should be used to warrant maximal differentiation among inbred lines. With respect to F. verticillioides infections, high FUM concentrations were found in grains from ears with minimal disease symptoms.

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Acknowledgments

This research was supported by the ‘Ministerium Ländlicher Raum Baden Württemberg’, grant no. 2005F03. The skilled technical assistance of N. Friedl, F. Mauch, and T. Schmid are gratefully acknowledged. We thank the mentioned colleagues (see Table 1) for sharing their isolates with us.

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Authors and Affiliations

  1. State Plant Breeding Institute, Universitaet Hohenheim (720), Fruwirthstrasse 21, 70593, Stuttgart, Germany

    T. Miedaner

  2. Institute of Plant Breeding, Seed Science, and Population Genetics, Universitaet Hohenheim (350), Fruwirthstrasse 21, 70593, Stuttgart, Germany

    C. Bolduan & A. E. Melchinger

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  1. T. Miedaner
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  2. C. Bolduan
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  3. A. E. Melchinger
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Correspondence to T. Miedaner.

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T. Miedaner and C. Bolduan contributed equally to this paper.

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Miedaner, T., Bolduan, C. & Melchinger, A.E. Aggressiveness and mycotoxin production of eight isolates each of Fusarium graminearum and Fusarium verticillioides for ear rot on susceptible and resistant early maize inbred lines. Eur J Plant Pathol 127, 113–123 (2010). https://doi.org/10.1007/s10658-009-9576-2

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