Abstract
Rice yellow mottle virus (RYMV) is a recent and major threat to rice production in Madagascar. A large scale screening of resistance to RYMV in rice germplasm in Madagascar was conducted by visual symptom scoring and virus-assessment through ELISA tests. The response to virus inoculation of 503 local or introduced rice accessions was assessed. Most of them were susceptible to RYMV. A few cultivars expressed partial resistance at a level similar to the partially resistant Oryza sativa japonica cv. Azucena. Only one O. sativa cultivar expressed high resistance characterised by a lack of symptoms and an undetectable level of virus. It was a Malagasy traditional indica cultivar, named Bekarosaka, which originated from the northwest of the country. It was selected by farmers for its field resistance to RYMV. The response of cv. Bekarosaka to four pathotypes of RYMV was similar to that of cv. Gigante, the only other highly resistant indica cultivar. The sequence of the middle domain of the eIF(iso)4G, the genetic determinant of Rymv1 resistance on chromosome 4, of cv. Bekarosaka was similar to that of cv. Gigante. Subsequently, cvs Bekarosaka and Gigante probably carried the same resistance allele Rymv1-2. Rymv1-2 resistance was efficient against isolates of the major strains of RYMV, but was readily overcome by a pathotype from the northwest of Madagascar.
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Abubakar, Z., Ali, F., Pinel, A., Traoré, O., N’Guessan, P., Notteghem, P., et al. (2003). Phylogeography of Rice yellow mottle virus in Africa. Journal of General Virology, 84, 733–743.
Albar, L., Bangratz-Reyser, M., Hébrard, E., Ndjiondjop, M., Jones, M., & Ghesquière, A. (2006). Mutations in the eiF(iso)4G translation initiation factor confer high resistance of rice to Rice yellow mottle virus. The Plant Journal, 47, 417–426.
Albar, L., Lorieux, M., Ahmadi, N., Rimbault, I., Pinel, A., Sy, A., et al. (1998). Genetic basis and mapping of the resistance to rice yellow mottle virus. I. QTLs identification and relationship between resistance and plant morphology. Theoretical and Applied Genetics, 97, 1145–1154.
Albar, L., Ndjiondjop, M., Esshak, Z., Berger, A., Pinel, A., Jones, M., et al. (2003). Fine genetic mapping of a gene required for Rice yellow mottle virus cell-to-cell movement. Theoretical and Applied Genetics, 107, 371–378.
Albar, L., Rakatomalala, M., Fargette, D., & Ghesquière, A. (2007). Molecular characterization of resistance to Rice yellow mottle virus in Bekarosaka, an indica variety from Madagascar. Rice Genetics Newsletter, 23, 84–88.
Bakker, W. (1974). Characterization and ecological aspect of Rice yellow mottle virus in Kenya. Wageningen: Agricultural University Press.
Fargette, D., Pinel, A., Abubakar, Z., Traoré, O., Brugidou, C., Fatogoma, S., et al. (2004). Inferring the evolutionary history of Rice yellow mottle virus from genomic, phylogenetic and phylogeographic studies. Journal of Virology, 78, 3252–3261.
Fargette, D., Pinel, A., Traoré, O., Ghesquière, A., & Konaté, G. (2002). Emergence of resistance-breaking isolates of rice yellow mottle virus during serial inoculations. European Journal of Plant Pathology, 108, 585–591.
Fauquet, C., & Thouvenel, J. (1977). Isolation of the Rice yellow mottle virus in Ivory Coast. Plant Disease Reporter, 61, 443–446.
Hébrard, E., Pinel-Galzi, A., Bersoult, A., Siré, C., & Fargette, D. (2006). Emergence of a resistance-breaking isolate of Rice yellow mottle virus during serial inoculations is due to a single substitution in the genome-linked viral protein VPg. Journal of General Virology, 87, 1369–1373.
Ioannidou, D., Lett, J., Pinel, A., Assigbetse, K., Brugidou, C., Ghesquière, A., et al. (2000). Responses of Oryza sativa japonica sub-species to infection with Rice yellow mottle virus. Physiological and Molecular Plant Pathology, 57, 177–188.
Ioannidou, D., Pinel, A., Brugidou, C., Albar, L., Ahmadi, N., Ghesquière, A., et al. (2003). Characterisation of the effects of a major QTL of partial resistance to Rice yellow mottle virus using near-isogenic-lines. Physiological and Molecular Plant Pathology, 63, 213–221.
Kochko de, A. (1988). Variabilité enzymatique des riz traditionnels malgaches Oryza sativa L. L’Agronomie Tropicale, 43, 203–208.
Konaté, G., Traoré, O., & Coulibaly, M. (1997). Characterization of Rice yellow mottle virus isolates in Sudano-Sahelian areas. Archives of Virology, 142, 1117–1124.
Kouassi, N., N’Guessan, P., Albar, L., Fauquet, C., & Brugidou, C. (2005). Distribution and characterization of Rice yellow mottle virus: a threat to African farmers. Plant Disease, 89, 124–133.
Ndjiondjop, M., Albar, N., Fargette, D., Fauquet, C., & Ghesquière, A. (1999). The genetic basis of high resistance to Rice yellow mottle virus (RYMV) in cultivars of two cultivated rice species. Plant Disease, 83, 931–935.
N’Guessan, P., Pinel, A., Caruana, M., Frutos, R., Sy, A., Ghesquière, A., et al. (2000). Evidence of the presence of two serotypes of Rice yellow mottle sobemovirus in Côte d’Ivoire. European Journal of Plant Pathology, 106, 167–178.
N’Guessan, P., Pinel, A., Sy, A., Ghesquière, A., & Fargette, D. (2001). Distribution, pathogenicity and interactions of two strains of Rice yellow mottle virus in forested and savanna zones of West-Africa. Plant Disease, 85, 59–64.
Pinel, A., N’Guessan, P., Bousalem, M., & Fargette, D. (2000). Molecular variability of geographically distinct isolates of Rice yellow mottle virus in Africa. Archives of Virology, 145, 1621–1638.
Pinel-Galzi, A., Rakotomalala, M., Sangu, E., Sorho, F., Kanyeka, Z., Traoré, O., et al. (2007). Theme and variations in the evolutionary pathways to virulence of an RNA plant virus species. PLoS Pathogens, 3, e180.
Rabary, E., Noyer, J., Beyayer, A., & Glaszman, J. (1989). Variabilité génétique du riz (Oryza sativa L.) à Madagascar: origine de types nouveaux. L’Agronomie Tropicale, 44, 305–312.
Rakotomalala, M. (2003). Case history on development and deployment of resistant varieties to control RYMV in Madagascar. In J. Hughes, & B. Odu (Eds.) Plant Virology in sub-Saharan Africa (pp. 423–431). Ibadan: International Institute for Tropical Agriculture.
Reckhaus, P., & Andriamasintseheno, H. (1997). Rice yellow mottle virus in Madagascar and its epidemiology in the northwest of the island. Journal of Plant Diseases and Protection, 104, 289–295.
Reckhaus, P., & Masajo, T. (1996). Panachure jaune du riz à Madagascar. Répartition-Epidémiologie-Lutte. Direction de la Protection des Végétaux. 57p.
Reckhaus, P., & Randrianangaly, S. (1990). Rice yellow mottle virus (RYMV) on rice in Madagascar. IRRI Newsletter, 15, 30.
Thottapilly, G., & Rossel, H. (1993). Evaluation of resistance to Rice yellow mottle virus in Oryza species. Indian Journal of Virology, 9, 65–73.
Traoré, O., Pinel, A., Hébrard, E., Gumedzoé, Y., Fargette, D., Traoré, A.,, et al. (2006). Occurrence of resistance-breaking isolates of Rice yellow mottle virus in West and Central Africa. Plant Disease, 90, 256–263.
Traoré, O., Sorho, F., Pinel, A., Abubakar, Z., Banwo, O., Maley, J., et al. (2005). Processes of diversification and dispersion of Rice yellow mottle virus inferred from large-scale and high-resolution phylogeographic studies. Molecular Ecology, 14, 2097–2110.
Virk, D., Dingh, D., Prasad, S., Gangwar, J., & Witcombe, J. (2003). Collaborative and consultative participatory plant breeding of rice for the rainfed uplands of eastern India. Euphytica, 132, 95–108.
Acknowledgements
We thank J. Aribi for technical assistance, E. Hébrard and N. Poulicard for helpful discussions, and J. M. Thresh for constructive criticisms of the manuscript. Funding by SIST and by the ECOGER action of the French National Programme ANR ‘Ecosphère continentale: processus, modélisation et risques environnementaux’ supported by INRA is acknowledged.
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Rakotomalala, M., Pinel-Galzi, A., Albar, L. et al. Resistance to Rice yellow mottle virus in rice germplasm in Madagascar. Eur J Plant Pathol 122, 277–286 (2008). https://doi.org/10.1007/s10658-008-9282-5
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DOI: https://doi.org/10.1007/s10658-008-9282-5