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Effect of leaf scald (Xanthomonas albilineans) on polyamine and phenolic acid metabolism of two sugarcane cultivars

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Abstract

Polyamine and phenolic acid levels as well as activities of some enzymes of their biosynthetic metabolism were examined in two sugarcane (Saccharum officinarum) cultivars differing in susceptibility to leaf scald, a disease caused by the bacterium, Xanthomonas albilineans. Juice obtained from both infected cultivars showed significantly increased levels of free putrescine and ornithine decarboxylase activity. However, the pathogen induced different changes in the two cultivars in subsequent metabolic steps. Whereas acid insoluble conjugated spermidine completely disappeared from the highly susceptible cv. C 439-52, an increase in acid insoluble conjugated polyamines was observed in the moderately susceptible cv. L 55-5. Phenolic acid metabolism also differed in the two cultivars. Since total phenolic acid content and phenylalanine ammonium lyase activity was greater in both cultivars after infection, distribution of phenolic acids between free or conjugated forms diverted into different pathways. The level of susceptibility of the two cultivars is discussed in terms of changes in these compounds.

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Abbreviations

CAD:

Cadaverine

HPLC:

High performance liquid chromatography

LDC:

Lysine decarboxylase

ODC:

Ornithine decarboxylase

PACs:

Phenolic acids

PAs:

Polyamines

PAL:

Phenylalanine-ammonium lyase

PCA:

Perchloric acid

PH-PAs:

Acid insoluble conjugated polyamines

PUT:

Putrescine

S-PAs:

Soluble polyamines

SH-PAs:

Acid soluble conjugated polyamines

SPD:

Spermidine

SPM:

Spermine

TNBS:

2,4,6-trinitrobenzene sulfonic acid

TNP:

Trinitrophenyl

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Acknowledgements

This work has been supported by a grant from the Ministerio de Educación y Ciencia (Spain) BFI2003-06234. We gratefully acknowledge the excellent technical assistance of Mrs. Raquel Alonso.

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Authors and Affiliations

  1. Department of Plant Physiology, Complutense University, 28040, Madrid, Spain

    Blanca Fontaniella, Carlos Vicente & María Estrella Legaz

  2. Department of Plant Physiology, Havana University, Havana, Cuba

    Roberto de Armas

Authors
  1. Blanca Fontaniella
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  2. Carlos Vicente
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  3. Roberto de Armas
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  4. María Estrella Legaz
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Correspondence to Blanca Fontaniella.

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Fontaniella, B., Vicente, C., de Armas, R. et al. Effect of leaf scald (Xanthomonas albilineans) on polyamine and phenolic acid metabolism of two sugarcane cultivars. Eur J Plant Pathol 119, 401–409 (2007). https://doi.org/10.1007/s10658-007-9172-2

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