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Non-targeted impact of cyantraniliprole residues on soil quality, mechanism of residue degradation, and isolation of potential bacteria for its bioremediation

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Abstract

Cyantraniliprole (CY), an anthranilic diamide insecticide widely used in grape farming for controlling various sucking pests, poses ecological concerns, particularly when applied as soil drenching due to the formation of more toxic and persistent metabolites. This study established the dissipation and degradation mechanisms of CY in grape rhizosphere soil using high-resolution Orbitrap-LC/MS analysis. The persistence of CY residues beyond 60 days was observed, with dissipation following biphasic first + first-order kinetics and a half-life of 15 to 21 days. The degradation mechanism of CY in the soil was elucidated, with identified metabolites such as IN-J9Z38, IN-JCZ38, IN-N7B69, and IN-QKV54. Notably, CY was found to predominantly convert to the highly persistent metabolite IN-J9Z38, raising environmental concerns. The impact of CY residues on soil enzyme activity was investigated, revealing a negative effect on dehydrogenase, alkaline phosphatase, and acid phosphatase activity, indicating significant implications for phosphorous mineralization and soil health. Furthermore, bacterial isolates were obtained from CY-enriched soil, with five isolates (CY3, CY4, CY9, CY11, and CY20) demonstrating substantial degradation potential, ranging from 66 to 92% of CY residues. These results indicate that the identified bacteria hold potential for commercial use in addressing pesticide residue contamination in soil through bioremediation techniques.

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Acknowledgements

The authors thank the Director, ICAR-National Research Centre for Grapes, Pune, India, and the Chairman, Agricultural & Processed Food Products Export Development Authority (APEDA), Ministry of Commerce, Government of India, New Delhi, India for providing infrastructural facilities to accomplish this project.

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  1. ICAR-National Research Centre for Grapes, Pune, Maharashtra, 412307, India

    Vrushali Bhanbhane, Sachin Ekatpure, Anita Pardeshi, Prabhavati Ghotgalkar, Pushpa Deore, Nasiruddin Shaikh, Anuradha Upadhyay & Ahammed Shabeer Thekkumpurath

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  1. Vrushali Bhanbhane
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Ahammed Shabeer Thekkumpurath, Anuradha Upadhyay: Conceptualization, Methodology, Design, and final manuscript; Vrushali Bhanbhane, Sachin Ekatpure, Anita Pardeshi, Prabhavati Ghotgalkar, Pushpa Deore, Nasiruddin Shaikh: Execution of experiment, statistical evaluation, and preliminary draft preparation.

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Bhanbhane, V., Ekatpure, S., Pardeshi, A. et al. Non-targeted impact of cyantraniliprole residues on soil quality, mechanism of residue degradation, and isolation of potential bacteria for its bioremediation. Environ Geochem Health 46, 171 (2024). https://doi.org/10.1007/s10653-024-01955-y

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