Abstract
The resiliency of plastic products against microbial degradation in natural environment often creates devastating changes for humans, plants, and animals on the earth’s surface. Biodegradation of plastics using indigenous bacteria may serve as a critical approach to overcome this resulting environmental stress. In the present work, a polyethylene degrading bacterium Alcaligenes faecalis strain ISJ128 (Accession No. MK968769) was isolated from partially degraded polyethylene film buried in the soil at plastic waste disposal site. The biodegradation studies were conducted by employing various methods such as hydrophobicity assessment of the strain ISJ128, measurement of viability and total protein content of bacterial biofilm attached to the polyethylene surface. The proliferation of bacterial cells on polyethylene film, as indicated by high growth response in terms of protein content (85.50 µg mL−1) and viability (1010 CFU mL−1), proposed reasonable suitability of our strain A. faecalis ISJ128 toward polyethylene degradation. The results of biodegradation assay revealed significant degradation (10.40%) of polyethylene film within a short period of time (i.e., 60 days), whereas no signs of degradation were seen in control PE film. A. faecalis strain ISJ128 also demonstrated a removal rate of 0.0018 day−1 along with half-life of 462 days. The scanning electron microscope (SEM) and Fourier transform infrared (FTIR) spectroscopy studies not only displayed changes on polyethylene surface but also altered level of intensity of functional groups and an increase in the carbonyl indexes justifying the degradation of polyethylene film due to bacterial activity. In addition, the secondary structure prediction (M fold software) of 16SrDNA proved the stable nature of the bacterial strain, thereby reflecting the profound scope of A. faecalis strain ISJ128 as a potential degrader for the eco-friendly disposal of polyethylene waste.
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All authors contributed equally to perform the analysis and to collect experimental data under the supervision of KKG. The idea of performing this research and designing of methodology came from KKG and DD. Data analysis was conducted by KKG and DD. The draft manuscript was prepared by DD with the assistance of KKG, HC, KS, HDMC, and KKS. Manuscript was critically reviewed by KKG, APM, HDMC, and HC. All authors read and approved the final manuscript.
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Devi, D., Gupta, K.K., Chandra, H. et al. Biodegradation of low-density polyethylene (LDPE) through application of indigenous strain Alcaligenes faecalis ISJ128. Environ Geochem Health 45, 9391–9409 (2023). https://doi.org/10.1007/s10653-023-01590-z
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DOI: https://doi.org/10.1007/s10653-023-01590-z