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Effect of chicken manure on soil microbial community diversity in poultry keeping areas

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Abstract

The poultry industry is generating a significant amount of waste from chicken droppings that are abundant in microbes as well as macro- and micronutrients suitable for manure. It has the potential to improve the microbial activity and nutrient dynamics in the soil, ultimately improving soil fertility. The present study aimed to investigate the effect of chicken droppings manure (CDM) on the diversity of the soil microbiome in the free walking chicken’s area located in Stefanidar, Rostov Region, Russia. The data obtained were compared with 16 s rRNA from control samples located not far from the chicken's free-walking area, but not in direct contact with the droppings. Effect of CDM on the physicochemical characteristics of the soil and changes in its microbial diversity were assessed by employing the metagenomic approaches and 16 s rRNA-based taxonomic assessment. The alpha and beta diversity indices revealed that the application of the CDM significantly improved the soil microbial diversity. The 16S taxonomical analysis confirmed Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Planctomycetes as abundant bacterial phylum. It also revealed the increase in the total number of the individual operational taxonomic unit (OTU) species, a qualitative indicator of the rich microbial community. The alpha diversity confirmed that the significant species richness of the soil is associated with the CDM treatment. The increased OTUs represent the qualitative indicator of a community that has been studied up to the depth of 5–20 cm of the CDM treatment range. These findings suggested that CDM-mediated microbial richness are believed to confer the cycling of carbon, nitrogen, and sulfur, along with key soil enzymes such as dehydrogenases and catalase carbohydrate-active enzymes. Hence, the application of CDM could improve soil fertility by nutrient cycling caused by changes in soil microbial dynamics, and it could also be a cost-effective sustainable means of improving soil health.

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Acknowledgements

This study was funded by the Government of the Russian Federation (grant number No. 075-15-2022-285) and by the Strategic Academic Leadership Program of the Southern Federal University ("Priority 2030").

Funding

This study was funded by the Government of the Russian Federation (Grant Number No. 075-15-2019-1880).

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Authors and Affiliations

  1. Southern Federal University, Rostov-on-Don, Russian Federation, 344090

    Tatiana Minkina, Svetlana Sushkova, Yanina Delegan, Anzhelika Bren, Maria Mazanko, Vishnu D. Rajput, Saglara Mandzhieva, Evgeniya V. Prazdnova & Anuj Ranjan

  2. Don State Technical University, 1 Gagarina Square, Rostov-on-Don, Russian Federation, 344002

    Anzhelika Bren, Dmitry Rudoy, Vereshak Elena & Galina Zelenkova

  3. Federal Research Center, “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Pushchino, Moscow Region, Russian Federation, 142290

    Yanina Delegan, Yulia Kocharovskaya & Andrey Filonov

  4. The Federal State Budget Educational Institution of Higher Education, Pushchino State Institute of Natural Science, Pushchino, Moscow Region, Russian Federation, 142290

    Yulia Kocharovskaya

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  1. Tatiana Minkina
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  2. Svetlana Sushkova
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Contributions

TM, SS, SM, and VDR conceptualized the manuscript ; TM supervised the study; TM, SS, SM, YD, AB, MM, and YK done experimentation; TM, SS, VDR, and AR, AB, MM, YK, RKS, DR, and EP did original draft preparation, review and editing; SS,VDR, VE, and GZ were involved in software support; TM, SS, and SM visualized and investigated the study; SS and TM performed project administration; SS, TM, and SM contributed to funding acquisition.

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Correspondence to Anuj Ranjan.

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Minkina, T., Sushkova, S., Delegan, Y. et al. Effect of chicken manure on soil microbial community diversity in poultry keeping areas. Environ Geochem Health 45, 9303–9319 (2023). https://doi.org/10.1007/s10653-022-01447-x

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