Abstract
The assessment of arsenic (As) bioavailability from contaminated matrices is a crucial parameter for reducing the uncertainty when estimating exposure for human health risk assessment. In vivo assessment of As utilising swine is considered an appropriate model for human health risk assessment applications as swine are remarkably similar to humans in terms of physiology and As metabolism. While limited in vivo As bioavailability data is available in the literature, few details have been provided regarding technical considerations for performing in vivo assays. This paper describes, with examples, surgical, experimental design and analytical issues associated with performing chronic and acute in vivo swine assays to determine As bioavailability in contaminated soil and food.
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Abedin, M. J., Cresser, M. S., Meharg, A. A., Feldmann, J., & Cotter-Howells, J. (2002). Arsenic accumulation and metabolism in rice (Oryza sativa L.). Environmental Science and Technology, 36, 962–968.
Agilent Technologies. (2006). Determination of heavy metals in whole blood by ICP-MS. Agilent Technologies publication number 5988-0533EN.
Akter, K. F., Chen, Z., Smith, L., Davey, D., & Naidu, R. (2005). Speciation of arsenic in groundwater samples: a comparative study of CE-UV, HG-AAS and LC-ICP-MS. Talanta, 68, 406–415.
Bain, S. A. F., Ting, J., Simeonovic, C. J., & Wilson, J. D. (1991). Technique of venous catheterization for sequential blood sampling from the pig. Journal of Investigative Surgery, 4, 103–107.
Casteel, S. W., Brown, L. D., Dunsmore, M. E., Weis, C. P., Henningsen, G. M., Hoffman, E., Brattin, W., Hammon, T. L. (1997). Relative bioavailability of arsenic in mining wastes. Document control no. 4500-88-AORH. U.S. Environmental Protection Agency, Region 8, Denver, CO.
Csanaky, I., & Gregus, Z. (2002). Species variations in the biliary and urinary excretion of arsenate, arsenite and their metabolites. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 131, 355–365.
Freeman, G. B., Johnson, J. D., Killinger, J. M., Liao, S. C., Davis, A. O., Ruby, M. V., et al. (1993). Bioavailability of arsenic in soil impacted by smelter activities following oral administration in rabbits. Fundamental and Applied Toxicology, 21, 83–88.
Freeman, G. B., Schoof, R. A., Ruby, M., Davis, A. O., Dill, S. C., Liao, S. C., et al. (1995). Bioavailability of arsenic in soil and house dust impacted by smelter activities following oral administration in cynomolgus monkeys. Fundamental and Applied Toxicology, 28, 215–222.
Gregus, Z., Gyurasics, A., & Csanaky, I. (2000). Biliary and urinary excretion of inorganic arsenic: monomethylarsonous acid as a major biliary metabolite in rats. Toxicology Science, 56, 18–25.
Groen, K., Vaessen, H., Kliest, J. J. G., de Boer, J. L. M., Ooik, T. V., Timmerman, A., et al. (1994). Bioavailability of inorganic arsenic from bog ore-containing soil in the dog. Environmental Health Perspectives, 102, 182–184.
Guha Mazumder, D. N., Haque, R., Ghosh, N., De, B. K., Santra, A., Chakraborti, D., et al. (1998). Arsenic levels in drinking water and the prevalence of skin lesions in West Bengal, India. International Journal of Epidemiology, 27, 871–877.
Guo, H. R., Chiang, H. S., Hu, H., Lipsitz, S. R., & Monson, R. R. (1997). Arsenic in drinking water and incidence of urinary cancers. Epidemiology, 8, 545–550.
Gyurasics, A., Varga, R., & Gregus, Z. (1991). Glutathione-dependent biliary excretion of arsenic. Biochemistry and Pharmacology, 42, 465–468.
Holliman, C. J., Kenfield, K., Nutter, E., Saffle, J. R., & Warden, G. D. (1982). Technique for acute subpubic catheterisation of urinary bladder in the pig. American Journal of Veterinary Research, 43, 1056–1057.
Hughes, M. F., Devesa, V., Adair, B. M., Styblo, M., Kenyon, E. M., & Thomas, D. J. (2005). Tissue dosimetry, metabolism and excretion of pentavalent and trivalent monomethylated arsenic in mice after oral administration. Toxicology and Applied Pharmacology, 208, 186–197.
Juhasz, A. L., Smith, E., Weber, J., Rees, M., Rofe, A., Kuchel, T., et al. (2006). In vivo assessment of arsenic bioavailability in rice and its significance for human health risk assessment. Environmental Health Perspectives, 114, 1826–1831.
Juhasz, A. L., Smith, E., Weber, J., Rees, M., Rofe, A., Kuchel, T., et al. (2007). Comparison of in vivo and in vitro methodologies for the assessment of arsenic bioavailability in contaminated soils. Chemosphere. doi: 10.1016/j.chemosphere.2007.05.018.
Kelly, M. E., Brauning, S. E., Schoof, R. A., & Ruby, M. V. (2002). Assessing oral bioavailability of metals in soil. Ohio: Battelle Press.
Lien, H. C., Tsai, T. F., Lee, Y. Y., & Hsiao, C. H. (2001). Merkel cell carcinoma and chronic arsenicism. Journal of the American Academy of Dermatology, 41, 641–643.
Mandal, B. K., & Suzuki, K. T. (2002). Arsenic round the world: a review. Talanta, 58, 201–235.
Ng, J. C., Kratzmann, S. M., Qi, L., Crawley, H., Chiswell, B., & Moore, M. (1998). Speciation and absolute bioavailability: risk assessment of arsenic-contaminated sites in a residential suburb in Canberra. Analyst, 123, 889–892.
Rahman, M. M., Chowdhury, U. K., Mukherjee, S. C., Mondal, B. K., Paul, K., Lodh, D., et al. (2001). Chronic arsenic toxicity in Bangladesh and West Bengal, India: a review and commentary. Journal of Toxicology: Clinical Toxicology, 39, 683–700.
Roberts, S. M., Weimar, W. R., Vinson, J. R. T., Munson, J. W., & Bergeron, R. J. (2002). Measurement of arsenic bioavailability in soil using a primate model. Toxicology Science, 67, 303–310.
Rodriguez, R. R., Basta, N. T., Casteel, S. W., & Pace, L. W. (1999). An in vitro gastrointestinal method to estimate bioavailable arsenic in contaminated soils and solid media. Environmental Science and Technology, 33, 642–649.
Ruby, M., Schoof, R., Brattin, W., Goldade, M., Post, G., Harnois, M., et al. (1999). Advances in evaluating the oral bioavailability of inorganics in soil for use in human health risk assessment. Environmental Science and Technology, 33, 3697–3705.
Smith, E., Naidu, R., & Alston, A. M. (1998). Arsenic in the soil environment: a review. Advances in Agronomy, 64, 149–195.
Thurmon, J. C., Nelson, D. R., Bevill, R. F., Harrnigton, G. W., & Magee, D. N. (1987). Surgical procedure for chronic bilary sample collection in pigs. American Journal of Veterinary Research, 48, 988–989.
USEPA. (1998). Method 3051A, microwave assisted acid digest of sediments, sludges, soils and oils. In USEPA methods, pp 3051A/1-24.
Weis, C. P., & LaVelle, J. M. (1991). Characteristics to consider when choosing an animal model for the study if lead bioavailability. Chemical Speciation and Bioavailability, 3, 113–119.
Acknowledgements
This research was funded through the Australian Research Council Linkage Grant Scheme, Grant number LP0347301. In vivo assays were approved and conducted according to application No. 17/02 of the Institute for Medical and Veterinary Science Animal Ethics Committee. The authors would like to acknowledge the support of the Centre for Environmental Risk Assessment and Remediation (University of South Australia), Centre for Pharmaceutical Studies (University of South Australia), and the Institute for Medical and Veterinary Science for this research.
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Rees, M., Sansom, L., Rofe, A. et al. Principles and application of an in vivo swine assay for the determination of arsenic bioavailability in contaminated matrices. Environ Geochem Health 31 (Suppl 1), 167–177 (2009). https://doi.org/10.1007/s10653-008-9237-y
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DOI: https://doi.org/10.1007/s10653-008-9237-y