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Molecular characterization of complement 9 in Epinephelus coioides and differential expression analysis of classical complement genes following Vibrio alginolyticus challenge

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Abstract

Vibrio alginolyticus is posting an increasing threat to survival of grouper. Classical complement cascade can trigger initiation of immunity, while complement 9 (C9) is a major complement molecule involved in final step of membrane attack complex (MAC) formation. In this study, full-length EcC9 contained an ORF sequence of 1779 bp, encoding a polypeptide of 592 amino acids. A high-level expression of EcC9 mRNA was observed in liver. Following vibrio challenge, increased expression levels of EcC1q, EcBf/C2, EcC4, EcC6, EcC7 and EcC9 mRNA were detected in liver and kidney. These results implied that elevated expression level of classical complement pathway (CCP) and terminal complement components (TCCs) may assess toxicological effect of V. alginolyticus.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China, China (grant no. 31902363) and the Doctoral Publishing Fund of Hunan Normal University, China (grant nos. 368002).

Funding

This research was funded by National Natural Science Foundation of China, China (grant no. 31902363) and the Doctoral Publishing Fund of Hunan Normal University, China (grant nos. 368002).

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Authors and Affiliations

  1. State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, PR China

    Sheng-Wei Luo

  2. Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou, 510631, PR China

    Wei Wei

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  1. Sheng-Wei Luo
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Luo, SW., Wei, W. Molecular characterization of complement 9 in Epinephelus coioides and differential expression analysis of classical complement genes following Vibrio alginolyticus challenge. Ecotoxicology 29, 837–845 (2020). https://doi.org/10.1007/s10646-020-02252-x

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