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Effects of feeding time on complement component C7 expression in Pelteobagrus vachellii subject to bacterial challenge

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Abstract

Shifting the feeding time for fish from daytime to nighttime could alter their digestive behavior, disturb their metabolism, and may affect immune-related genes. This study aimed to clone complement component C7 and analyze the different expression of C7 mRNA in fish fed during either the day or at night and then challenged with Aeromonas hydrophila infection. The Pv-C7 cDNA of Pelteobagrus vachellii contained 2 647 bp with an open reading frame encoding a protein of 818 amino acids. Multiple sequences analysis indicated that Pv-C7 included eight domains, which was similar to results for other species. Quantitative PCR analysis showed that Pv-C7 was mainly expressed in the liver, spleen, intestine and head kidney tissues of healthy P. vachellii. Quantitative PCR analysis showed that C7 mRNA transcript in the liver, spleen and head kidney also increased significantly when the fish were fed at nighttime (20:00). In addition, the expression of Pv-C7 mRNA significantly increased with A. hydrophila challenge in the liver (48–96 h), spleen and head kidney (12–96 h) tissues of P. vachellii. Pv-C7 mRNA expression of the fish fed at nighttime showed significant higher than that in the fish fed at day time at 12–48 h in head kidney and 12–24 h in spleen. This study indicates that altering the feeding time from daytime to nighttime could increase Pv-C7 mRNA expression, and feeding time may affect the immune response involving C7.

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Authors and Affiliations

  1. College of Life Science, Sichuan Normal University, Chengdu, 610101, China

    Ting Shao  (邵婷) & Fanglan Ge  (葛芳兰)

  2. College of Life Science, Neijiang Normal University, Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang, 641000, China

    Ting Shao  (邵婷), Chuanjie Qin  (覃川杰), Huiguo Duan  (段辉国), Dengyue Yuan  (袁登越), Zhengyong Wen  (文正勇) & Jun Wang  (王钧)

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  1. Ting Shao  (邵婷)
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  2. Chuanjie Qin  (覃川杰)
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  3. Huiguo Duan  (段辉国)
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  4. Dengyue Yuan  (袁登越)
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  5. Zhengyong Wen  (文正勇)
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  6. Jun Wang  (王钧)
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  7. Fanglan Ge  (葛芳兰)
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Correspondence to Chuanjie Qin  (覃川杰).

Additional information

Supported by the National Natural Science Foundation of China (No. 31402305), the Natural Science Foundation of Sichuan Province (No. 2017JY0161), and the Educational Commission of Sichuan Province of China (No. 14ZA0249)

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Shao, T., Qin, C., Duan, H. et al. Effects of feeding time on complement component C7 expression in Pelteobagrus vachellii subject to bacterial challenge. J. Ocean. Limnol. 36, 2358–2367 (2018). https://doi.org/10.1007/s00343-019-7270-6

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  • DOI: https://doi.org/10.1007/s00343-019-7270-6

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