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Water extracts from winery by-products as tobacco defense inducers

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Abstract

Water extracts from winery by-products exhibited significant plant defense inducer properties. Experiments were conducted on three marc extracts containing various amounts of polyphenols and anthocyanins. Infiltration of red, white and seed grape marc extracts into tobacco leaves induced hypersensitive reaction-like lesions with cell death evidenced by Evans Blue staining. The infiltration zones and the surrounding areas revealed accumulation of autofluorescent compounds under UV light. Leaf infiltration of the three winery by-product extracts induced defense gene expression. The antimicrobial PR1, β-1,3-glucanase PR2, and chitinase PR3 target genes were upregulated locally in tobacco plants following grape marc extract treatments. The osmotin PR5 transcripts accumulated as well in red marc extract treated-tobacco leaves. Overall, the winery by-product extracts elicited an array of plant defense responses making the grape residues a potential use of high value compounds.

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Acknowledgments

This work had financial support provided by grants from FUI: Fonds Unique Interministériel. We thank Céline Sac for her help in growing tobacco plants and Dominique Marcon for the tobacco leaf images.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. UMR 547 PIAF, Clermont Université, Université Blaise Pascal, 63000, Clermont-Ferrand, France

    Razik Benouaret, Eric Goujon, Gérard Ledoigt & Pascale Goupil

  2. UMR 547 PIAF, INRA, 63100, Clermont-Ferrand, France

    Razik Benouaret, Eric Goujon, Gérard Ledoigt & Pascale Goupil

  3. Institut de Chimie de Clermont-Ferrand, Clermont Université, Université Blaise Pascal, BP 10448, 63000, Clermont-Ferrand, France

    Aurélien Trivella & Claire Richard

  4. Institut de Chimie de Clermont-Ferrand, UMR 6296, CNRS, 63171, Aubiere, France

    Aurélien Trivella & Claire Richard

  5. Laboratoires Goëmar, avenue du Général Patton, 35400, Saint Malo, France

    Jean-Marie Joubert & Aude Mery-Bernardon

Authors
  1. Razik Benouaret
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  2. Eric Goujon
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  3. Aurélien Trivella
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  4. Claire Richard
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  5. Gérard Ledoigt
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  6. Jean-Marie Joubert
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  8. Pascale Goupil
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Correspondence to Pascale Goupil.

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Benouaret, R., Goujon, E., Trivella, A. et al. Water extracts from winery by-products as tobacco defense inducers. Ecotoxicology 23, 1574–1581 (2014). https://doi.org/10.1007/s10646-014-1298-3

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  • DOI: https://doi.org/10.1007/s10646-014-1298-3

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