Abstract
Organ coefficients (including kidney, testis, liver and spleen coefficient) and abnormal sperm rate were used in our study to reflect the exposure to the Yangzte River water. The concentrations of total dissolved metals and semi-volatile organic compounds in tap and source water were measured by ICP-OES and GC–MS, respectively. After mice were fed with purified water (CK), Nanjing tap water (NJT) and Nanjing source water (NJS) for 90 day, the individual and organs (including kidney, testis, liver and spleen) of each mouse were weighted. And abnormal sperm types (such as hook less, banana-like form, amorphous, folded and two tails) were determined by microscope. The results showed that significant differences of liver coefficient between experimental group (NJT, NJS) and control group (CK) were observed; furthermore liver coefficient is positive correlation with the concentrations of total dissolved metals. However, no significant differences of abnormal sperm rates between experimental group (NJT, NJS) and control group (CK) were noted. So liver coefficient might be more sensitive than other organ coefficients to reflect the exposure to tap water and source water, while abnormal sperm rate could not be used to reveal the exposure to them.
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References
Cheng SP (2003) Heavy metal pollution in China: origin, pattern and control. Environ Sci Pollut Res 10(3):192–198. doi:10.1065/espr2002.11.141.1
Cheng X-L, Cheng Y-H, Liu H, Lu W-Q (2005) Effect of chlorinated drinking water extracts on lipid peroxidation in HEPG2 cells. Acta Hydrobiol Sin 29(1):38–42
Feng C, Xia X, Shen Z, Zhou Z (2007) Distribution and sources of polycyclic aromatic hydrocarbons in Wuhan section of the Yangtze River, China. Environ Monit Assess 133(1–3):447–458. doi:10.1007/s10661-006-9599-5
Gao J, Liu L, Liu X, Zhou H, Huang S, Wang Z (2008a) Levels and spatial distribution of chlorophenols 2,4-dichlorophenol, 2,4,6-trichlorophenol, and pentachlorophenol in surface water of China. Chemosphere 71(6). doi: 10.1016/j.chemosphere.2007.10.018
Gao J, Liu L, Liu X, Zhou H, Wang Z, Huang S (2008b) Concentration level and geographical distribution of nitrobenzene in Chinese surface waters. J Environ Sci (China) 20(7). doi: 10.1016/s1001-0742(08)62129-4
Han FA, Chen J, Jiang ZF, Chen LS, Ji WL (2013) Volatile and semi-volatile organic compounds in the lower Yangtze River and surface waters of three Chinese provinces. Pol J Environ Stud 22(3):683–690
Li Y-Q, Wu Y-L, Chen Y-G, Kong Z-M (2006) Genotoxicity evaluation and a primary risk assessment of organic pollutants in the drinking water sources of Nanjing, China. J Environ Sci (China) 18(5). doi: 10.1016/s1001-0742(06)60026-0
Lu WQ, Chen D, Wu XJ, Liu AL, Liu H, Wu JJ, Mersch-Sundermann V (2004) DNA damage caused by extracts of chlorinated drinking water in human derived liver cells (HepG2). Toxicology 198(1–3):351–357. doi:10.1016/j.tox.2004.02.011
MOHPRC, SACPRC (2006) Standards fo drinking water quality. vol GB 5749-2006
Shen L, Wu JY, Lin GF, Shen JH, Westendorf J, Huehnerfuss H (2003) The mutagenic potentials of tap water samples in Shanghai. Chemosphere 52(9):1641–1646. doi:10.1016/s0045-6535(03)00504-6
Shi W, Zhang F, Zhang X, Su G, Wei S, Liu H, Cheng S, Yu H (2011) Identification of trace organic pollutants in freshwater sources in Eastern China and estimation of their associated human health risks. Ecotoxicology 20(5):1099–1106. doi:10.1007/s10646-011-0671-8
USEPA (1992) Method 3005a acid digestion of waters for total recoverable or dissolved metals for analysis by FLAA or ICP spectroscopy. http://www.epa.gov/waste/hazard/testmethods/sw846/pdfs/3005a.pdf. Accessed 22 March 2014
Wang F, Sha Y-J, Xia X-H, Liu H (2008) Distribution characteristics of phthalic acid esters in the Wuhan section of the Yangtze River. Huan jing ke xue 29(5):1163–1169
Wu D, Li Y, Tang B, Fang F, Ding S-M, Yang X, Yuan J-L (2007) Effects of butyl benzyl phthalate on enzymes related to energy metabolism in testis of mice. Asian J Ecotoxicol 2(4):440–444
Wu B, Zhang X, Zhang X, Yasun A, Zhang Y, Zhao D, Ford T, Cheng S (2009a) Semi-volatile organic compounds and trace elements in the Yangtze River source of drinking water. Ecotoxicology 18(6):707–714. doi:10.1007/s10646-009-0331-4
Wu B, Zhang XX, Zhang XL, Yasun ASJ, Zhang Y, Zhao DY, Ford T, Cheng SP (2009b) Semi-volatile organic compounds and trace elements in the Yangtze River source of drinking water. Ecotoxicology 18(6):707–714. doi:10.1007/s10646-009-0331-4
Wu B, Zhao DY, Zhang Y, Zhang XX, Cheng SP (2009c) Multivariate statistical study of organic pollutants in Nanjing reach of Yangtze River. J Hazard Mater 169(1–3):1093–1098. doi:10.1016/j.jhazmat.2009.04.065
Wu J, Hu G, Wang X, Li D, Yu H, Han X (2010) The reproductive toxicity of organic compounds extracted from drinking water sources on sprague dawley rats: an in vitro study. Environ Toxicol 25(3):284–293. doi:10.1002/tox.20498
Wu J, Wang X, Ying F, Hu G, Wang X, Li D, Yu H, Han X (2011) In vitro assessment of reproductive toxicity on rats induced by organic contaminants of source water. Ecotoxicol Environ Saf 74(6):1756–1764. doi:10.1016/j.ecoenv.2011.03.016
Xiao-rong D, Xiao-ming Y, Yi LU, Kun-peng MA, Jie BAO, Hao-tian Z, Fei T (2010) Comparison of the biotoxicity of organic compounds in source and tap water from the Yangtze and Han River. China Environ Sci 30(2):263–268
Xu C, Zhang J-J, Chen J-A, Cao B, Shu W-Q, Cao J (2010) Evaluation of ovotoxicity in female mice caused by organic extracts in tap water from Jialing river in Chongqing, China. Birth Defects Res B Dev Reprod Toxicol 89(1):26–33. doi:10.1002/bdrb.20219
Yun SF, Tian XY, Cheng SP, Zhang Y, Li AM, Zhang LB, Zhang XX, Chen L, Wu B, Guo LQ, Shi YZ (2011) Serum biochemical analysis to indicate pathogenic risk on mouse Mus musculus exposure to source of drinking water. Ecotoxicology 20(5):1078–1082. doi:10.1007/s10646-011-0669-2
Zhang Q, Zhou J, Wang K, Zhao R, Ao S, Wu Q, Wang H (2007) Toxicity variation of organic extracts in drinking water from two kinds of source waters. J Southeast Univ Nat Sci Ed 37(1):148–152
Zhang XX, Zhang ZY, Ma LP, Liu N, Wu B, Zhang Y, Li AM, Cheng SP (2010) Influences of hydraulic loading rate on SVOC removal and microbial community structure in drinking water treatment biofilters. J Hazard Mater 178(1–3):652–657. doi:10.1016/j.jhazmat.2010.01.135
Zhang R, Wang W, Shi X, Yu X, Li M, Xiao L, Cui Y (2011) Health risk of semi-volatile organic pollutants in Wujin river inflow into Taihu Lake. Ecotoxicology 20(5):1083–1089. doi:10.1007/s10646-011-0682-5
Zhang Y, Zhang X, Wu B, Cheng S (2012) Evaluating the transcriptomic and metabolic profile of mice exposed to source drinking water. Environ Sci Technol 46(1):78–83. doi:10.1021/es201369x
Zhao D, Cheng S, Wu B, Zhang Y, Zhang X, Li W, Cui Y (2009) Toxicity of the Yangtze River source of drinking water on reproductive system of male mice (Mus musculus). Ecotoxicology 18(6):729–735. doi:10.1007/s10646-009-0337-y
Zhao D, Chen Y, Zhou K, Cheng S, Ma T, Jiang C, Yan W, Zhu L, Gu X, Zhu X, Wu B, Zhang Y, Zhang X (2011) Reproductive toxicity in male mice exposed to Nanjing City tap water. Ecotoxicology 20(5):1057–1064. doi:10.1007/s10646-011-0644-y
Zheng L, Zhao A-H, Wang W, Qia L-Y, Jia S-Z, Kou L-J, Li F-X, Wu X-N, Wang G-Z (2010) Preliminary evaluation on immunotoxicity of repeat administration with BCG-CpG-DNA in Mice. Chin J Biol 23(9):922–925
Acknowledgments
This study was supported by National Natural Science Foundation of China (31072217), State Key Laboratory of Environmental Chemistry and Ecotoxicology (KF2009-19) and Natural Science Foundation of Tibet Autonomous Region.
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Zhang, R., Zhang, L., Jiang, D. et al. Mouse organ coefficient and abnormal sperm rate analysis with exposure to tap water and source water in Nanjing reach of Yangtze River. Ecotoxicology 23, 641–646 (2014). https://doi.org/10.1007/s10646-014-1228-4
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DOI: https://doi.org/10.1007/s10646-014-1228-4