Abstract
Background
Circular RNAs (circRNAs) can act as promoters or inhibitors in cancer progression. Has_circ_0006948 (circ_0006948) was reported to aggravate the malignant behaviors of esophageal carcinoma (EC).
Aims
This study focused on investigating the molecular mechanism of circ_0006948 in EC progression.
Methods
The quantitative real-time polymerase chain reaction was performed to detect the expression of circ_0006948, microRNA-4262 (miR-4262) and fibronectin type III domain containing 3B (FNDC3B). Cell growth analysis was conducted by Cell Counting Kit-8 and colony formation assays. Cell migration and invasion were assessed by transwell assay. Epithelial–mesenchymal transition (EMT)-associated proteins and FNDC3B protein expression were assayed using western blot. Dual-luciferase reporter and RNA pull-down assays were performed to validate the target combination. Xenograft tumor assay was used for investigating the role of circ_0006948 in vivo.
Results
Circ_0006948 was upregulated in EC tissues and cells. Downregulating the expression of circ_0006948 or FNDC3B repressed cell growth, migration, invasion and EMT in EC cells. Target analysis indicated that miR-4262 was a target for circ_0006948 and FNDC3B was a downstream gene for miR-4262. Moreover, circ_0006948 could affect the expression of FNDC3B via sponging miR-4262. The effects of si-circ_0006948#1 on EC cells were partly restored by miR-4262 inhibition or FNDC3B overexpression. In addition, circ_0006948 also facilitated EC tumorigenesis in vivo by targeting the miR-4262/FNDC3B axis.
Conclusion
Taken together, circ_0006948 functioned as an oncogenic factor in EC by the miR-4262-mediated FNDC3B expression regulation.
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Supplementary Fig. 1
The anti-tumor regulation of si-circ_0006948#1 was reversed by miR-4262 inhibitor. (A–K) Cellular assays were performed by CCK-8 (A–B) and colony formation assay (C–D) for cell growth, transwell assay for cell migration (F–G) and invasion (H–I), and western blot for EMT-related protein detection after transfection of si-NC, si-circ_0006948#1, si-circ_0006948#1+anti-NC or si-circ_0006948#1+anti-miR-4262. **P < 0.01, ***P < 0.001.
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Yue, M., Liu, Y., Zuo, T. et al. Circ_0006948 Contributes to Cell Growth, Migration, Invasion and Epithelial–Mesenchymal Transition in Esophageal Carcinoma. Dig Dis Sci 67, 492–503 (2022). https://doi.org/10.1007/s10620-021-06894-7
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DOI: https://doi.org/10.1007/s10620-021-06894-7