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A Shift from Colon- to Ileum-Predominant Bacteria in Ileal-Pouch Feces Following Total Proctocolectomy

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Abstract

Background

We previously investigated fecal flora of the pouch after total proctocolectomy using terminal restriction fragment polymorphism analysis. Although the results of the cluster analysis demonstrated clearly that bacterial populations, including an unidentified bacteria generating a 213-bp PCR fragment, moved toward a colon-like community in the pouch, it did not track changes in the individual species of fecal bacteria.

Aims

The aim of the present study was to estimate genome copy number of ten bacterial species, clusters, groups, or subgroups (including the bacteria generating 213-bp fragment in the previous study) in feces samples from pouches at various times following ileostomy closure.

Methods

A total of 117 stool samples were collected from patients with ulcerative colitis after surgery as well as healthy volunteers. We used real-time polymerase chain reaction of the 16S rRNA gene to estimate genome copy numbers for the nine bacterial populations and the bacteria generating 213-bp fragment after identification by DNA sequencing.

Results

We demonstrated a time-dependent increase in the number of anaerobic and colon-predominant bacteria (such as Clostridium coccoides, C. leptum, Bacteroides fragilis and Atopobium) present in proctocolectomy patients after stoma closure. In contrast, numbers of ileum-predominant bacterial species (such as Lactobacillus and Enterococcus faecalis) declined.

Conclusions

Our data confirm previous findings that fecal flora in the pouch after total proctocolectomy changes significantly, and further demonstrate that the number and diversity of ileal bacteria decreases while a more colon-like community develops. The present data are essential for the future analysis of pathological conditions in the ileal pouch.

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References

  1. Fukushima K, Haneda S, Takahashi K, et al. Molecular analysis of colonic transformation in the ileum after total colectomy in rats. Surgery. 2006;140:93–99.

    Article  PubMed  Google Scholar 

  2. Kohyama A, Ogawa H, Funayama Y, et al. Bacterial population moves toward a colon-like community in the pouch after total proctocolectomy. Surgery. 2009;145:435–447.

    Article  PubMed  Google Scholar 

  3. Matsuki T, Watanabe K, Fujimoto J, Takada T, Tanaka R. Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces. Appl Environ Microbiol. 2004;70:7220–7228.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Rinttila T, Kassinen A, Malinen E, Krogius L, Palva A. Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol. 2004;97:1166–1177.

    Article  CAS  PubMed  Google Scholar 

  5. Ohge H, Furne JK, Springfield J, Rothenberger DA, Madoff RD, Levitt MD. Association between fecal hydrogen sulfide production and pouchitis. Dis Colon Rectum. 2005;48:469–475.

    Article  PubMed  Google Scholar 

  6. Roediger WE, Duncan A, Kapaniris O, Millard S. Sulphide impairment of substrate oxidation in rat colonocytes: a biochemical basis for ulcerative colitis? Clin Sci Lond. 1993;85:623–627.

    CAS  PubMed  Google Scholar 

  7. Nadkarni MA, Martin FE, Jacques NA, Hunter N. Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology. 2002;148:257–266.

    CAS  PubMed  Google Scholar 

  8. Fite A, Macfarlane GT, Cummings JH, et al. Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction. Gut. 2004;53:523–529.

    Article  CAS  PubMed  Google Scholar 

  9. Menard JP, Fenollar F, Henry M, Bretelle F, Raoult D. Molecular quantification of Gardnerella vaginalis and Atopobium vaginae loads to predict bacterial vaginosis. Clin Infect Dis. 2008;47:33–43.

    Article  CAS  PubMed  Google Scholar 

  10. Bartosch S, Fite A, Macfarlane GT, McMurdo ME. Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota. Appl Environ Microbiol. 2004;70:3575–3581.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Langendijk PS, Schut F, Jansen GJ, et al. Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl Environ Microbiol. 1995;61:3069–3075.

    CAS  PubMed Central  PubMed  Google Scholar 

  12. Coffey JC, Rowan F, Burke J, Dochery NG, Kirwan WO, O’Connell PR. Pathogenesis of and unifying hypothesis for idiopathic pouchitis. Am J Gastroenterol. 2009;104:1013–1023.

    Article  PubMed  Google Scholar 

  13. Nasmyth DG, Godwin PG, Dixon MF, Williams NS, Johnston D. Ileal ecology after pouch-anal anastomosis or ileostomy. A study of mucosal morphology, fecal bacteriology, fecal volatile fatty acids, and their interrelationship. Gastroenterology. 1989;96:817–824.

    CAS  PubMed  Google Scholar 

  14. Smith FM, Coffey JC, Kell MR, O’Sullivan M, Redmond HP, Kirwan WO. A characterization of anaerobic colonization and associated mucosal adaptations in the undiseased ileal pouch. Colorectal Dis. 2005;7:563–570.

    Article  CAS  PubMed  Google Scholar 

  15. Sato S, Fukushima K, Naito H, et al. Induction of 11beta-hydroxysteroid dehydrogenase type 2 and hyperaldosteronism are essential for enhanced sodium absorption after total colectomy in rats. Surgery. 2005;137:75–84.

    Article  PubMed  Google Scholar 

  16. Swidsinski A, Ladhoff A, Pernthaler A, et al. Mucosal flora in inflammatory bowel disease. Gastroenterology. 2002;122:44–54.

    Article  PubMed  Google Scholar 

  17. Marteau P, Lepage P, Mangin I, et al. Review article: gut flora and inflammatory bowel disease. Aliment Pharmacol Ther. 2004;20:18–23.

    Article  PubMed  Google Scholar 

  18. Lepage P, Seksik P, Sutren M, et al. Biodiversity of the mucosa-associated microbiota is stable along the distal digestive tract in healthy individuals and patients with IBD. Inflamm Bowel Dis. 2005;11:473–480.

    Article  PubMed  Google Scholar 

  19. Sokol H, Seksik P, Rigottier-Gois L, et al. Specificities of the fecal microbiota in inflammatory bowel disease. Inflamm Bowel Dis. 2006;12:106–111.

    Article  PubMed  Google Scholar 

  20. Christl SU, Eisner HD, Dusel G, Kasper H, Scheppach W. Antagonistic effects of sulfide and butyrate on proliferation of colonic mucosa: a potential role for these agents in the pathogenesis of ulcerative colitis. Dig Dis Sci. 1996;41:2477–2481.

    Article  CAS  PubMed  Google Scholar 

  21. Duffy M, O’Mahony L, Coffey JC, et al. Sulfate-reducing bacteria colonize pouches formed for ulcerative colitis but not for familial adenomatous polyposis. Dis Colon Rectum. 2002;45:384–388.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

A part of the results were generated by using the facilities of the Biomedical Research Core of Tohoku University Graduate School of Medicine. This work was supported in part by Health and Labour Sciences Research Grants for research on intractable diseases from the Ministry of Health, Labour and Welfare of Japan.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Division of Molecular and Surgical Pathophysiology, Graduate School of Medicine, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan

    Manami Hinata & Kouhei Fukushima

  2. Division of Biological Regulation and Oncology, Department of Surgery, Graduate School of Medicine, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, 980-8574, Japan

    Atsushi Kohyama, Hitoshi Ogawa, Sho Haneda, Kazuhiro Watanabe, Hideyuki Suzuki, Chikashi Shibata & Iwao Sasaki

  3. Division of Colorectal and Anal Diseases, Tohoku Rosai Hospital, 4-3-21, Dainohara, Aoba-ku, Sendai, 981-8563, Japan

    Yuji Funayama & Ken-ichi Takahashi

  4. Laboratory of Gastrointestinal Tract, Department of Regenerative Medicine, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan

    Kouhei Fukushima

Authors
  1. Manami Hinata
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  2. Atsushi Kohyama
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  3. Hitoshi Ogawa
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  4. Sho Haneda
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  5. Kazuhiro Watanabe
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  6. Hideyuki Suzuki
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  7. Chikashi Shibata
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  8. Yuji Funayama
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  9. Ken-ichi Takahashi
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  10. Iwao Sasaki
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  11. Kouhei Fukushima
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Corresponding author

Correspondence to Kouhei Fukushima.

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Hinata, M., Kohyama, A., Ogawa, H. et al. A Shift from Colon- to Ileum-Predominant Bacteria in Ileal-Pouch Feces Following Total Proctocolectomy. Dig Dis Sci 57, 2965–2974 (2012). https://doi.org/10.1007/s10620-012-2165-9

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  • DOI: https://doi.org/10.1007/s10620-012-2165-9

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