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Digestive Diseases and Sciences

, Volume 52, Issue 9, pp 2265–2270 | Cite as

Helicobacter pylori May Play a Contributory Role in the Pathogenesis of Primary Sclerosing Cholangitis

  • Alyssa M. KrasinskasEmail author
  • Yuan Yao
  • Parmjeet Randhawa
  • Maria P. Dore
  • Antonia R. Sepulveda
Original Paper

Abstract

Helicobacter pylori (H. pylori) DNA has been identified in human livers and has been implicated in chronic liver disease and liver cancer. To better understand the role of H pylori in primary sclerosing cholangitis (PSC), 25 patients with end-stage PSC and 31 controls were studied. Genomic DNA was extracted from microdissected hilar hepatic ducts of liver explants and was amplified for H pylori DNA. Serum was tested for H pylori antibodies. Helicobacter DNA was detected in 9 of the 56 (16%) patients by 16SrRNA PCR (an additional case [for a total of 18%] was antibody positive). Seven of the 9 cases identified by polymerase chain reaction were positive for the CagA gene, confirming they were H pylori. Seven of the 25 (28%) patients with PSC and 3 of the 31 (9.7%) controls were positive for Helicobacter (P=.087). H pylori DNA was detected in microdissected hilar biliary epithelium in more PSC patients than controls, supporting the hypothesis that bile reflux from the duodenum into the biliary tract might carry H pylori organisms into the proximal biliary system, possibly contributing to PSC development and/or progression in some patients.

Keywords

Helicobacter pylori Primary sclerosing cholangitis Liver explant Polymerase chain reaction Microdissection 

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Alyssa M. Krasinskas
    • 1
    • 4
    Email author
  • Yuan Yao
    • 1
  • Parmjeet Randhawa
    • 2
  • Maria P. Dore
    • 3
  • Antonia R. Sepulveda
    • 1
  1. 1.Department of Pathology, Division of Anatomic PathologyUniversity of PittsburghPittsburghUSA
  2. 2.Department of Pathology, Division of Transplant and Liver PathologyUniversity of PittsburghPittsburghUSA
  3. 3.Istituto di Clinica MedicaUniversity of SassariSassariItaly
  4. 4.Department of PathologyUniversity of Pittsburgh Medical Center, Presbyterian HospitalPittsburghUSA

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