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Effects of Motilin and Mitemcinal (GM-611) on Gastrointestinal Contractile Activity in Rhesus Monkeys In Vivo and In Vitro

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Abstract

Neither the presence of motilin receptors nor their action has been investigated in monkeys. The object of this study was to determine the effects of motilin and mitemcinal (GM-611), an erythromycin derivative, on the gastrointestinal tract in rhesus monkeys in vivo and in vitro. In in vivo investigations in conscious monkeys, both motilin and mitemcinal induced migrating motor complex-like contractions in the interdigestive state and also accelerated gastric emptying. In in vitro investigations, the presence of motilin receptors in the gastrointestinal tract was demonstrated by receptor binding assays. Motilin and mitemcinal contracted isolated duodenum strips in a concentration-dependent manner. In conclusion, rhesus monkeys may be useful for studying the physiological and pharmacological roles of the motilin agonistic mechanism because they show reactivity to motilin both in vivo and in vitro.

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Acknowledgements

The authors wish to thank Mr. Toyokazu Matsu-ura, Mr. Hajime Sano, Ms. Akiko Hiura, Ms. Mayumi Miyaguchi, and Ms. Yuriko Seto-Ozaki for their expert assistance in the animal experiments. The authors also thank Dr. Paul Langman and Mr. Geoffrey Read for their editorial assistance.

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Authors and Affiliations

  1. Fuji-Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd., Gotemba, Shizuoka, 412-8513, Japan

    Kenji Yogo, Ken-ichi Ozaki, Hisanori Takanashi & Masao Koto

  2. Gunma University, Maebashi, Gunma, 371-8512, Japan

    Zen Itoh

  3. The Kitasato Institute, Minato-ku, Tokyo, 108-8642, Japan

    Satoshi Ōmura

  4. Targeted Disease Areas Department, Chugai Pharmaceutical Co., Ltd., 1-1 Nihonbashi-Muromachi 2-Chome, Chuo-ku, Tokyo, 103-8324, Japan

    Hisanori Takanashi

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  1. Kenji Yogo
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  2. Ken-ichi Ozaki
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  3. Hisanori Takanashi
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  4. Masao Koto
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  5. Zen Itoh
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  6. Satoshi Ōmura
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Correspondence to Hisanori Takanashi.

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Yogo, K., Ozaki, Ki., Takanashi, H. et al. Effects of Motilin and Mitemcinal (GM-611) on Gastrointestinal Contractile Activity in Rhesus Monkeys In Vivo and In Vitro. Dig Dis Sci 52, 3112–3122 (2007). https://doi.org/10.1007/s10620-006-9672-5

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  • DOI: https://doi.org/10.1007/s10620-006-9672-5

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