Abstract
β-lactoglobulin (BLG) and ε-polylysine (PL) were bound by using microbial transglutaminase. Dextran (Dex) was further conjugated to the BLG-PL conjugate by the Maillard reaction. Confirmation of conjugation was carried out by SDS-PAGE. From the results of isoelectric focusing, it was revealed that the isoelectric point of the BLG-PL conjugate was shifted to the basic side as compared with native BLG. Immunogenicity of BLG in BALB/c mice was lowered by conjugation with PL and further lowered by conjugation with Dex. By conjugation with PL and PL-Dex, antibacterial activity against Staphylococcus aureus was endowed to BLG. Because the conjugation method in this study is a safe method, it is valuable in that it can be applicable to food processing.
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The data underlying this article are available in the article and also from the corresponding author upon request.
Abbreviations
- BLG:
-
β-Lactoglobulin
- PL:
-
Epsilon polylysine
- SDS-PAGE:
-
Sodium dodecyl sulfate poly acrylamide gel electrophoresis
- CBB:
-
Coomassie brilliant blue
- IEF:
-
Isoelectric focusing
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Acknowledgements
We thank CHISSO CORPORATION (Tokyo, Japan) for presenting ε-polylysine. We thank Ajinomoto Co. (Tokyo,Japan) for presenting Activa TG-K (E.C.2.3.2.13). This work was supported in part by JSPS KAKENHI Grant Number JP22580126.
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TY: Conceptualization, Data curation, Writing manuscript, Funding acquisition, SK: Conceptualization, Data curation, Writing manuscript, RI, KH, YM: Data curation, Writing manuscript, MH: Conceptualization, Writing manuscript, Funding acquisition.
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Yoshida, T., Ikeda, R., Kawai, S. et al. Reduced immunogenicity and endowed antimicrobial activity in β-lactoglobulin by preparing edible bioconjugate. Cytotechnology 75, 39–47 (2023). https://doi.org/10.1007/s10616-022-00558-8
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DOI: https://doi.org/10.1007/s10616-022-00558-8