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NOL6, a new founding oncogene in human prostate cancer and targeted by miR-590-3p

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Abstract

We identified a new human prostate cancer oncogene, nucleolar protein 6 (NOL6), and screened for microRNAs that interfere with its expression in prostate cancer cells. A NOL6 shRNA plasmid was constructed and packaged into lentivirus to infect PC-3 cells. The ability of cell proliferation was evaluated by cell counting and colony formation. Cell cycle progression and apoptosis of PC-3 cells were detected by flow cytometry. A retrieval database was used to screen possible target microRNAs, and the effect of target miRNA overexpression on PC-3 cells was observed. The results showed that after NOL6 gene knockdown, PC-3 cell mitosis was blocked, proliferation was decreased, and the number of apoptotic cells were increased. The microRNA, hsa-miR-590-3p, that can regulate the NOL6 gene expression was identified. Overexpression of miR-590-3p in PC-3 cells by synthetic mimics resulted in abnormal mitosis, decreased cell proliferation, and an increase in apoptosis. In summary, we identified NOL6 as a novel oncogene in the human prostate cancer PC-3 cell line. The miRNA miR-590-3p interferes with NOL6 expression making it a potential treatment for prostate cancer.

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Acknowledgements

This study was supported by Science and Technology Suppor Program of Jiangxi Province (20141BBG70052).

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  1. Degang Dong and Mei Song contributed equally to this work.

Authors and Affiliations

  1. School of Life Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, People’s Republic of China

    Degang Dong

  2. School of Science and Technology, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, People’s Republic of China

    Mei Song

  3. College of Chinese Medicine, Hunan University of Traditional Chinese Medicine, Changsha, 410208, People’s Republic of China

    Xiaoli Wu

  4. Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, 330006, People’s Republic of China

    Wanchun Wang

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  1. Degang Dong
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  2. Mei Song
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  3. Xiaoli Wu
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  4. Wanchun Wang
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Correspondence to Wanchun Wang.

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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Dong, D., Song, M., Wu, X. et al. NOL6, a new founding oncogene in human prostate cancer and targeted by miR-590-3p. Cytotechnology 72, 469–478 (2020). https://doi.org/10.1007/s10616-020-00394-8

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  • DOI: https://doi.org/10.1007/s10616-020-00394-8

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