Abstract
In mammals, interferon-inducible transmembrane proteins (IFITMs) prevent infections by various enveloped viruses. The expression of IFITMs in chicken was herein examined in the adult and embryonic organs using a quantitative reverse-transcription-polymerase chain reaction. The results obtained revealed that IFITM3 was expressed at a higher level than IFITM1, 2 and 5, in both embryonic and adult organs. However, the expression levels of IFITMs in embryonic organs were less than 5 % of those in adult lungs. Among the embryonic tissues examined, primordial germ cells (PGCs) at day 2.5 expressed relatively higher levels of IFITM3. IFITM3 expression levels were 1.5-fold higher in the chicken cell line DF-1 than in PGCs. The knockdown of IFITM3 in DF-1 cells by siRNA increased the infectivity of a vesicular stomatitis virus G protein-pseudotyped lentiviral vector, suggesting that lower levels of IFITM3 are still sufficient to restrict this viral vector.
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References
Bailey CC, Zhong G, Huang IC, Farzan M (2014) IFITM-family proteins: the cell’s first line of antiviral defense. Annu Rev Virol 1:261–283
D’Costa S, Pardue SL, Petitte JN (2001) Comparative development of avian primordial germ cells and production of germ line chimeras. Avian Poult Biol Rev 12:151–168
Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92
Houdebine LM (2000) Transgenic animal bioreactors. Transgenic Res 9:305–320
Ito T, Suzuki Y, Takada A, Kawamoto A, Otsuki K, Masuda H, Yamada M, Suzuki T, Kida H, Kawaoka Y (1997) Differences in sialic acid-galactose linkages in the chicken egg amnion and allantois influence human influenza virus receptor specificity and variant selection. J Virol 71:3357–3362
Kamihira M, Ono K, Esaka K, Nishijima K, Kigaku R, Komatsu H, Yamashita T, Kyogoku K, Iijima S (2005) High-level expression of single-chain Fv-Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector. J Virol 79:10864–10874
Kamihira M, Kawabe Y, Shindo T, Ono K, Esaka K, Yamashita T, Nishijima K, Iijima S (2009) Production of chimeric monoclonal antibodies by genetically manipulated chickens. J Biotechnol 141:18–25
Kodama D, Nishimiya D, Iwata K, Yamaguchi K, Yoshida K, Kawabe Y, Motono M, Watanabe H, Yamashita T, Nishijima K, Kamihira M, Iijima S (2008) Production of human erythropoietin by chimeric chickens. Biochem Biophys Res Commun 367:834–839
Kues WA, Niemann H (2004) The contribution of farm animals to human health. Trends Biotechnol 22:286–294
Kyogoku K, Yoshida K, Watanabe H, Yamashita T, Kawabe Y, Motono M, Nishijima K, Kamihira M, Iijima S (2008) Production of recombinant tumor necrosis factor receptor/Fc fusion protein by genetically manipulated chickens. J Biosci Bioeng 105:454–459
Motono M, Ohashi T, Nishijima K, Iijima S (2008) Analysis of chicken primordial germ cells. Cytotechnology 57:199–205
Motono M, Yamada Y, Hattori Y, Nakagawa R, Nishijima K, Iijima S (2010) Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector. J Biosci Bioeng 109:315–321
Nishijima K, Iijima S (2013) Transgenic chickens. Dev Growth Differ 55:207–216
Perreira JM, Chin CR, Feeley EM, Brass AL (2013) IFITMs restrict the replication of multiple pathogenic viruses. J Mol Biol 425:4937–4955
Rudolph NS (1999) Biopharmaceutical production in transgenic livestock. Trends Biotechnol 17:367–374
Smith EC, Popa A, Chang A, Masante C, Dutch RE (2009) Viral entry mechanisms: the increasing diversity of paramyxovirus entry. FEBS J 276:7217–7227
Smith SE, Gibson MS, Wash RS, Ferrara F, Wright E, Temperton N, Kellam P, Fife M (2013) Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssaviruses in vitro. J Virol 87:12957–12966
Yount JS, Karssemeijer RA, Hang HC (2012) S-palmitoylation and ubiquitination differentially regulate interferon-induced transmembrane protein 3 (IFITM3)-mediated resistance to influenza virus. J Biol Chem 287:19631–19641
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This work was supported by JSPS KAKENHI Grant Number 26289312.
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Kidani, S., Okuzaki, Y., Kaneoka, H. et al. Expression of interferon-inducible transmembrane proteins in the chicken and possible role in prevention of viral infections. Cytotechnology 69, 477–484 (2017). https://doi.org/10.1007/s10616-016-9958-1
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DOI: https://doi.org/10.1007/s10616-016-9958-1