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Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity

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Abstract

A major drawback with cancer chemotherapy is its severe toxic effects on non-target tissues. Assessment of natural products for their protective effect against anticancer drugs-induced toxicity is gaining importance in cancer biology. The present study was aimed at assessing the protective effect of hydroethanolic extract of Indian propolis (HEIP) against mitomycin C (MMC)-induced genotoxicity and cytotoxicity. Swiss albino mice were injected with various doses of HEIP (100, 200, 300, 400, 600 and 800 mg/kg b. wt., i.p) 1 h prior to MMC (8 mg/kg, i.p.) injection. The geno- and cyto-toxicities were evaluated in mice by performing bone marrow micronucleus and TUNEL assays. In vitro antioxidant and lipid peroxidation inhibitory assays were carried out to understand the mechanism of the protective effects. The significant increase in the frequency of micronculeated cells (12.51 ± 0.48), apoptotic cells (23.43 ± 1.86) and reduction in P/N ratio (0.69 ± 0.04) compared with control indicated the potential geno- and cytotoxic effects of MMC in bone marrow. Pretreatment with HEIP resulted in the significant recovery of the toxic effects induced by MMC. HEIP at 400 mg/kg b. wt. was found to be the optimum dose imparting the maximum protective effects. The in vitro antioxidant and lipid peroxidation inhibitory assays suggest that the extract possesses substantial free radical scavenging activities. In conclusion, HEIP possesses substantial geno- and cyto-protective properties against MMC, which could be mediated through efficient free radical scavenging and inhibitory effect on lipid peroxidation.

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Authors and Affiliations

  1. Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, Karnataka, 576 104, India

    Sandhya Kumari, Sujith Raj Salian, Guruprasad Kalthur & Satish Kumar Adiga

  2. Department of PG Studies and Research in Biosciences, Mangalore University, Managalagangothri, Karnataka, 574 199, India

    Prashantha Naik & B. L. Vishma

  3. Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, 576 104, India

    Raviraj Anand Devkar, Saleemulla Khan & Srinivas Mutalik

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  1. Sandhya Kumari
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  2. Prashantha Naik
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  3. B. L. Vishma
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  8. Guruprasad Kalthur
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Correspondence to Guruprasad Kalthur.

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Kumari, S., Naik, P., Vishma, B.L. et al. Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity. Cytotechnology 68, 1789–1800 (2016). https://doi.org/10.1007/s10616-015-9931-4

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  • DOI: https://doi.org/10.1007/s10616-015-9931-4

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