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Proteomic profiling of human placenta-derived mesenchymal stem cells upon transforming LIM mineralization protein-1 stimulation

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Abstract

Human placenta-derived mesenchymal stem cells (hPDMSCs) can differentiate into different types of cells and thus have tremendous potential for cell therapy and tissue engineering. LIM mineralization protein-1 (LMP-1) plays an important role in osteoblast differentiation, maturation and bone formation. To determine a global effect of LMP-1 on hPDMSCs, we designed a study using a proteomic approach combined with adenovirus-mediated gene transfer of LMP-1 to identify LMP-1-induced changes in hPDMSCs on proteome level. We have generated proteome maps of undifferentiated hPDMSCs and LMP-1 induced hPDMSCs. Two dimensional gel electrophoresis revealed 22 spots with at least 2.0-fold changes in expression and 15 differently expressed proteins were successfully identified by MALDI-TOF-MS. The proteins regulated by LMP-1 included cytoskeletal proteins, cadmium-binding proteins, and metabolic proteins, etc. The expression of some identified proteins was confirmed by further Western blot analyses. Our results will play an important role in better elucidating the underlying molecular mechanism in LMP-1 included hPDMSCs differentiation into osteoblasts.

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Acknowledgments

This work was supported by the 10th Science and Technology Supporting Program project from Zhenjiang city (SH2010043) and Natural Science Foundation of Jilin Province (20130101017jc).

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Authors and Affiliations

  1. Stomatology Hospital, Jilin University, Changchun, 130021, People’s Republic of China

    Zhen Zhu, Zhihui Liu, Ming Bi, Taoyuan Yang & Jingyun Wang

  2. Stomatology Hospital of Zhenjiang, Zhenjiang, 212002, People’s Republic of China

    Jingyue Liu

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  1. Zhen Zhu
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  2. Zhihui Liu
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  3. Jingyue Liu
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Correspondence to Jingyun Wang.

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Zhu, Z., Liu, Z., Liu, J. et al. Proteomic profiling of human placenta-derived mesenchymal stem cells upon transforming LIM mineralization protein-1 stimulation. Cytotechnology 67, 285–297 (2015). https://doi.org/10.1007/s10616-013-9684-x

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