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RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from Dicentrarchus labrax

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Abstract

Continuous cell lines could provide an important tool for studying epidemiology, toxicology, cellular physiology and the host–pathogen interactions. Random amplified polymorphic deoxyribonucleic acid analysis by PCR (RAPD-PCR) was used for the molecular characterization of Dicentrarchus labrax embryonic cells (DLEC) as a possible tool to detect DNA alterations in environmental genotoxic studies. We studied the DNA pattern of the DLEC fish cell line, a fibroblast-like cell line derived from European sea bass. From a total of 15 primers only six showed good discriminatory power for the amplification process on DNA samples collected from cells by three different methods (organic extraction, salting-out method and chelating agent extraction). The results obtained show that the cell line chosen for this study could be used as a possible tool for the detection of potential genotoxicity of numerous chemical compounds.

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Acknowledgments

The authors would like to express their gratitude to Dr. Antony Bridgewood for his assistance in revising the English of this manuscript.

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Authors and Affiliations

  1. Department of Environmental, Biological and Pharmaceutical Sciences and Technology, Second University of Naples, Caserta, Italy

    L. Rocco, I. V. Valentino & V. Stingo

  2. Interuniversity Center Reach (IC-REACH), University of Naples Federico II, Naples, Italy

    L. Rocco & V. Stingo

  3. Department of Environmental Sciences, Tuscia University, Viterbo, Italy

    G. Scapigliati

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  1. L. Rocco
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  2. I. V. Valentino
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  3. G. Scapigliati
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  4. V. Stingo
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Correspondence to L. Rocco.

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Rocco, L., Valentino, I.V., Scapigliati, G. et al. RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from Dicentrarchus labrax . Cytotechnology 66, 383–393 (2014). https://doi.org/10.1007/s10616-013-9586-y

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