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Enhancement of antibody production by the addition of Coenzyme-Q10

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Abstract

Recently, there has been a growing demand for therapeutic monoclonal antibodies (MAbs) on the global market. Because therapeutic MAbs are more expensive than low-molecular-weight drugs, there have been strong demands to lower their production costs. Therefore, efficient methods to minimize the cost of goods are currently active areas of research. We have screened several enhancers of specific MAb production rate (SPR) using a YB2/0 cell line and found that coenzyme-Q10 (CoQ10) is a promising enhancer candidate. CoQ10 is well known as a strong antioxidant in the respiratory chain and is used for healthcare and other applications. Because CoQ10 is negligibly water soluble, most studies are limited by low concentrations. We added CoQ10 to a culture medium as dispersed nanoparticles at several concentrations (Q-Media) and conducted a fed-batch culture. Although the Q-Media had no effect on cumulative viable cell density, it enhanced SPR by 29%. In addition, the Q-Media had no effect on the binding or cytotoxic activity of MAbs. Q-Media also enhanced SPR with CHO and NS0 cell lines by 30%. These observations suggest that CoQ10 serves as a powerful aid in the production of MAbs by enhancing SPR without changing the characteristics of cell growth, or adversely affecting the quality or biological activity of MAbs.

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Abbreviations

MAbs:

Monoclonal antibodies

CoQ10 :

Coenzyme-Q10

Q-Media:

Culture media supplemented with dispersed nanoparticles of Q10

8OHdG:

8-hydroxy-2′-deoxyguanosine

SPR:

Specific MAb production rate (pg cell−1 d−1)

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Acknowledgments

We would like to thank Dr. Kazuyasu Nakamura, Ms. Masako Wakitani, and Mr. Noriyuki Takahashi for their expert analysis, and Mr. Hiroshi Takasugi, Dr. Kazuhisa Uchida, Dr. Jun Yamaya, and Dr. Mitsuo Sato for helpful discussions and encouragement.

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Author notes

  1. Naoto Sakai

    Present address: Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., Ltd., 100-1 Hagiwara-machi, Takasaki-shi, Gunma, 370-0013, Japan

  2. Motoi Aoki & Shinji Hosoi

    Present address: Kyowa Hakko Kirin Co., Ltd., 1-6-1 Ohte-machi, Chiyoda-ku, Tokyo, Japan

  3. Masamichi Koike

    Present address: BioWa, Inc., Princeton Commerce Center, 20 Emmons Drive, Suite C-10, Princeton, NJ, 08540, USA

  4. Kaori Wakamatsu

    Present address: Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu-shi, 376-8515, Japan

Authors and Affiliations

  1. Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., Ltd., 100-1 Hagiwara-machi, Takasaki-shi, Gunma, 370-0013, Japan

    Yoshinobu Konno

  2. Kyowa Hakko Kirin Co., Ltd., Tokyo Branch, 36-2 Nihonbashi Hakozaki-cho, Chuo-ku, Tokyo, Japan

    Masakazu Takagishi

  3. Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu-shi, 376-8515, Japan

    Yoshinobu Konno

Authors
  1. Yoshinobu Konno
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Correspondence to Yoshinobu Konno.

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Konno, Y., Aoki, M., Takagishi, M. et al. Enhancement of antibody production by the addition of Coenzyme-Q10 . Cytotechnology 63, 163–170 (2011). https://doi.org/10.1007/s10616-010-9330-9

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  • DOI: https://doi.org/10.1007/s10616-010-9330-9

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