Abstract
A human-mouse hybridoma clone 92-2 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was established. To detect antibody-binding sequences on Ara h1, we synthesized a series of peptides of the Ara h1 protein on a multi-pin apparatus for the pin-peptide ELISA. The 92-2 human monoclonal antibody was found to recognize a sequence of GREGEQEWGTPGSHVREETS. Further analysis with shorter pin-peptides with eight amino acid-long showed that the sequence of QEWGTPGS was an essential linear sequence of this epitope. When the QEW part of the sequence was replaced by alanine, the 92-2 monoclonal antibody did not bind to the substituted peptide, showing that those amino acids play an important role in the binding of the 92-2 monoclonal antibody.
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Shinmoto, H., Matsuo, Y., Naganawa, Y. et al. Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody 92-2. Cytotechnology 62, 307–311 (2010). https://doi.org/10.1007/s10616-009-9243-7
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DOI: https://doi.org/10.1007/s10616-009-9243-7