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Evaluation of inhibitory effect and apoptosis induction of Zyzyphus Jujube on tumor cell lines, an in vitro preliminary study

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Abstract

In the present study, water extract of dried fruit of Zyzyphus Jujube was tested for its possible anticancer effect and induction of apoptosis on human tumor cell lines, HEp-2, HeLa and Jurkat cell lines. The inhibitory effect of water extract of this fruit on cell proliferation was assessed by MTT colorimetric assay. The induction of apoptosis of this extract was analyzed by DNA fragmentation analysis. Zyzyphus Jujube extract showed inhibitory effects on mentioned cell lines. Jurkat leukemic line was found the most sensitive cells with IC50 of 0.1 μg mL−1. Our study also showed a typical DNA laddering in this cell line. The present study showed cytotoxic activity of Zyzyphus Jujube on tumor cells. Although Zyzyphus Jujube has useful compounds for medical applications.

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Abbreviations

BSA:

Bovine serum albumin

DMSO:

Dimethyl sulphoxide

FBS:

Fetal bovine serum

MTT:

3-(4,5-dimethylthiazoyl)-2,5-diphenyltetrazolium bromide

PBS:

Phosphate buffer saline

References

  • Beyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest 21(Supp 97):77–89

    Google Scholar 

  • Bown D (1995) Encyclopaedia of herbs and their uses. Dorling Kindersley, London

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Centers for Disease Control, Prevention (1995) Self-treatment with herbal and other plant-derived remedies-rural Mississippi. 1993. 204–207

  • Colic M, Pavelic K (2002) Molecular, cellular and medical aspects of the action of nutraceuticals and small molecules therapeutics: from chemoprevention to new drug development. Drugs Exp Clin Res 28(5):169–175

    CAS  Google Scholar 

  • Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W (1990) Trypan blue exclusion test of cell viability. Current protocols on immunology. Wiley, New York

    Google Scholar 

  • Cuendet M, Christov K, Lantvit DD et al (2004) Multiple myeloma regression mediated by bruceantin. Clin Cancer Res 10(3):1170–1179

    Article  CAS  Google Scholar 

  • Dorrie J, Sapala K, Zunino SJ (2001) Carnosol-induced apoptosis and downregulation of Bcl-2 in B-lineage leukemia cells. Cancer Lett 170(1):33–39

    Article  CAS  Google Scholar 

  • Dubois MG, Gilles KA, Hamilton JK, Rebers PA et al (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Duke JA, Ayensu ES (1985) Medicinal plants of china. Reference Publications, Institute of Chinese Medicine, S219–S224

  • Gonzales GF, Valerio LG (2006) Medicinal plants from Peru: a review of plants as potential agents against cancer. Anticancer Agents Med Chem 6(5):429–444

    Article  CAS  Google Scholar 

  • Herrmann M, Lorenz HM, Voll R et al (1994) A rapid and simple method for the isolation of apoptotic DNA fragments. Nucleic Acids Res 22(24):5506–5507

    Article  CAS  Google Scholar 

  • Him-Che Y (1985) Handbook of Chinese herbs and formulas. Institute of Chinese Medicine, S219–S224

  • Huang L, Pardee AB (1999) Beta-lapachone induces cell cycle arrest and apoptosis in human colon cancer cells. Mol Med 5(11):711–720

    CAS  Google Scholar 

  • Kim R, Tanabe K, Inoue H, Toge T (2002) Mechanism(s) of antitumor action in protracted infusion of low dose 5-fluorouracil and cisplatin in gastric carcinoma. Int J Oncol 20(3):549–555

    CAS  Google Scholar 

  • Lee SM, Min BS, Lee CG et al (2003) Cytotoxic triterpenoids from the fruits of Zizyphus jujuba. Planta Med 69(11):1051–1054

    Article  CAS  Google Scholar 

  • Malik A, Kuliev ZA, Akhmedov UA et al (2002) New oligomeric proanthocyanidine from Ziziphus jujuba. Chem Nat Compd 38(1):40–42

    Article  CAS  Google Scholar 

  • Mans DR, da Rocha AB, Schwartsmann G (2000) Anti-cancer drug discovery and development in Brazil: targeted plant collection as a rational strategy to acquire candidate anti-cancer compounds. Oncologist 5(3):185–198

    Article  CAS  Google Scholar 

  • Mignogna MD, Fedele S, Lo Russo L (2004) The World cancer report and the burden of oral cancer. Eur J Cancer Prev 13(2):139–142

    Article  CAS  Google Scholar 

  • Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  CAS  Google Scholar 

  • Ogbourne SM, Suhrbier A, Jones B et al (2004) Antitumor activity of 3-ingenyl angelate: plasma membrane and mitochondrial disruption and necrotic cell death. Cancer Res 64(8):2833–2839

    Article  CAS  Google Scholar 

  • Omidbaigi R, Daghighi S (2005) Effects of sucker age and transplanting time on the propagation of jujube tree. Paper presented at the III WOCMAP congress on medicinal and aromatic plants, Thailand

  • Rafter JJ (2002) Scientific basis of biomarkers and benefits of functional foods for reduction of disease risk: cancer. Br J Nutr 88(Suppl 2):S219–S224

    Article  CAS  Google Scholar 

  • Sampson W (2005) Studying herbal remedies. N Engl J Med 353(4):337–339

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Biotechnology Department, Vaccine and Serum Research Institute, Mashhad, Iran

    Fatemeh Vahedi, Mohsen Fathi Najafi & Kazem Bozari

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  1. Fatemeh Vahedi
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  2. Mohsen Fathi Najafi
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  3. Kazem Bozari
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Correspondence to Fatemeh Vahedi.

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Vahedi, F., Fathi Najafi, M. & Bozari, K. Evaluation of inhibitory effect and apoptosis induction of Zyzyphus Jujube on tumor cell lines, an in vitro preliminary study. Cytotechnology 56, 105–111 (2008). https://doi.org/10.1007/s10616-008-9131-6

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  • DOI: https://doi.org/10.1007/s10616-008-9131-6

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