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Use of cyanobacterial gas vesicles as oxygen carriers in cell culture

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Abstract

The gas vesicles isolated from the cells of filamentous cyanobacterium Anabaena flos-aquae were treated and sterilized with glutaraldehyde and then evaluated for their effectiveness as gas carriers in cell culture. Anchorage-dependent Vero cells were grown in a packed bed of microcarrier beads under the perfusion of Dulbecco’s Modified Eagle’s Medium with 1% serum. The culture medium supplemented with 1.8% (v/v) gas vesicles was found to support a 30% higher maximum glucose utilization rate than the same medium without gas vesicles. The gas vesicle suspension was confirmed to have no apparent effects on cell metabolism in T-flask cultures. The study results indicated that the gas vesicles, with high oxygen carrying capacity, can be used to increase the oxygen supply in cell culture systems.

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References

  • Adlercreutz P, Mattiasson B (1982) Oxygen supply to immobilized cells: Oxygen supply by hemoglobin or emulsions of perfluorochemicals. Eur J Appl Microbiol Biotechnol 16:165–170

    Article  CAS  Google Scholar 

  • Alvarez MM, Guzman A, Elias M (2005) Experimental visualization of mixing pathologies in laminar stirred tank bioreactors. Chem Eng Sci 60:2449–2457

    Article  CAS  Google Scholar 

  • Arrington SA, Zeleznik MJ, Ott DW, Ju L-K (2003) Effects of polyethyleneimine on cyanobacterium Anabaena flos-aquae during cell flocculation and flotation. Enz Microb Technol 32:290–293

    Article  CAS  Google Scholar 

  • Barnikol W, Poetzschke H (2003) Use of hemoglobin, myoglobin or their derivatives as oxygen carriers for the controlled oxygen supply of microorganisms, cell and tissue cultures and organs. Ger Offen 10220990

  • Brenner J, Huelser DF (1996) Production of tissue plasminogen activator (tPA) in two and three dimensionally growing cultures of Bowes melanoma cells. Biotechnol Bioeng 51:422–433

    Article  CAS  Google Scholar 

  • Curran SJ, Black RA (2005) Oxygen transport and cell viability in an annular flow bioreactor: Comparison of laminar couette and Taylor-vortex flow regimes. Biotechnol Bioeng 89:766–774

    Article  CAS  Google Scholar 

  • Handa-Corrigan A, Emery AN, Spier RE (1989) Effect of gas-liquid interfaces on the growth of suspended mammalian cells: mechanisms of cell damage by bubbles. Enz Microb Technol 11:230–235

    Article  CAS  Google Scholar 

  • Hayes PK, Buchholz B, Walsby AE (1992) Gas vesicles are strengthened by the outer-surface protein, GvpC. Arch Microbiol 157:229–234

    Article  CAS  Google Scholar 

  • Ho CS, Ju L-K, Baddour RF (1990) Enhancing penicillin fermentations by increased oxygen solubility through the addition of n-hexadecane. Biotechnol Bioeng 36:1110–1118

    Article  CAS  Google Scholar 

  • Imamura T, Crespi CL, Thilly WG, Brunengraber H (1982) Fructose as a carbohydrate source yields stable pH and redox parameters in microcarrier cell culture. Anal Biochem 124:353–358

    Article  CAS  Google Scholar 

  • Ju L-K, Armiger WB (1990) Enhancing oxygen transfer in surface-aerated bioreactors by stable foams. Biotechnol Prog 6:262–265

    Article  CAS  Google Scholar 

  • Ju L-K, Armiger WB (1992) Use of perfluorocarbon emulsions in cell culture. BioTechniques 12:258–263

    CAS  Google Scholar 

  • Ju L-K, Lee JF, Armiger WB (1991a) Enhancing oxygen transfer in bioreactors by perfluorocarbon emulsions. Biotechnol Prog 7:323–329

    Article  CAS  Google Scholar 

  • Ju L-K, Lee JF, Armiger WB (1991b) Effect of the interfacial surfactant layer on oxygen transfer through the oil/water phase boundary in perflurocarbon emulsions. Biotechnol Bioeng 37:505–511

    Article  CAS  Google Scholar 

  • Junker BH, Hatton TA, Wang DIC (1990) Oxygen transfer enhancement in aqueous/perfluorocarbon fermentation systems: I. Experimental observations. Biotechnol Bioeng 35:586–597

    Article  CAS  Google Scholar 

  • Kaiser GRRF, Monteiro SC, Gelain DP, Souza LF, Perry MLS, Bernard EA (2005) Metabolism of amino acids by cultured rat Sertoli cells. Metabol Clin Exp 54:515–521

    CAS  Google Scholar 

  • Kashyap S, Sundararajan A, Ju L-K (1998) Flotation characteristics of cyanobacterium Anabaena flos-aquae for gas vesicle production. Biotechnol Bioeng 60:636–641

    Article  CAS  Google Scholar 

  • Keipert PE (1995) Use of oxygent, a perfluorochemical-based oxygen carrier, as an alternative to intraoperative blood transfusion. Artif Cells Blood Subst Immobil Biotechnol 23:381–394

    CAS  Google Scholar 

  • Kim HW, Greenburg AG (2004) Artificial oxygen carriers as red blood cell substitutes: a selected review and current status. Artif Organs 28:813–828

    Article  CAS  Google Scholar 

  • King AT, Mulligan BJ, Lowe KC (1987) Perfluorochemicals and cell culture. Bio/Technol 7:1037–1042

    Google Scholar 

  • Ma N, Chalmers JJ, Aunins JG, Zhou W, Xie L (2004) Quantitative studies of cell-bubble interactions and cell damage at different pluronic F-68 and cell concentrations. Biotechnol Prog 20:1183–1191

    Article  CAS  Google Scholar 

  • Maranga L, Cunha A, Clemente J, Cruz P, Carrondo MJT (2004) Scale-up of virus-like particles production: effects of sparging, agitation and bioreactor scale on cell growth, infection kinetics and productivity. J Biotechnol 107:55–64

    Article  CAS  Google Scholar 

  • Nagase K, Kohori F, Sakai K, Nishide H (2005) Rearrangement of hollow fibers for enhancing oxygen transfer in an artificial gill using oxygen carrier solution. J Memb Sci 254:207–217

    Article  CAS  Google Scholar 

  • Nahapetian AT, Thomas JN, Thilly WG (1986) Optimization of environment for high density vero cell culture: effect of dissolved oxygen and nutrient supply on cell growth and changes in metabolites. J Cell Sci 81:65–103

    CAS  Google Scholar 

  • Oh SKW, Nienow AW, Al-Rubeai M, Emery AN (1987) On the evaluation of gas-liquid interfacial effects on hybridoma viability in bubble column bioreactors. J Biotechnol 12:45–62

    Article  Google Scholar 

  • Pithon-Curi TC, Pires de Melo M, Curi R (2004) Glucose and glutamine utilization by rat lymphocytes, monocytes and neutrophils in culture: A comparative study. Cell Biochem Funct 22:321–326

    Article  CAS  Google Scholar 

  • Reiss JG, Le Blanc M (1982) Solubility and transport phenomena in perfluorochemicals relevant to blood substitution and other biomedical applications. Pure Appl Chem 54:2383–2406

    Google Scholar 

  • Remy B, Deby-Dupont G, Lamy M (1999) Red blood cell substitutes: fluorocarbon emulsions and haemoglobin solutions. Brit Med Bull 55:277–298

    Article  CAS  Google Scholar 

  • Shima M, Seino Y, Tanaka H, Kurose H, Ishida M, Yabuuchi H, Kodama H (1988) Microcarriers facilitate mineralization in MC3T3-E1 cells. Calcif Tissue Int 43:19–25

    Article  CAS  Google Scholar 

  • Sundararajan A, Ju L-K (2000a) Evaluation of oxygen permeability of gas vesicles from cyanobacterium Anabaena flos-aquae. J Biotechnol 77:151–156

    Article  CAS  Google Scholar 

  • Sundararajan A, Ju L-K (2000b) Glutaraldehyde treatment of proteinaceous gas vesicles from cyanobacterium anabaena flos-aquae. Biotechnol Prog 16:1124–1128

    Article  CAS  Google Scholar 

  • Thilly WG, Barngrover D, Thomas JN (1982) Microcarriers and the problem of high density cell culture. In: From gene to protein: translation into biotechnology. Academic Press, New York, pp 75–103

  • Walsby AE (1969) The permeability of blue-green algal gas vacuole membranes to gas. Proc R Soc Lond Ser B 173:235–255

    Article  Google Scholar 

  • Walsby AE (1982) The elastic compressibility of gas vesicles. Proc R Soc Lond B 216:355–368

    Google Scholar 

  • Walsby AE (1994) Gas vesicles. Microbiol Rev 58:94–144

    CAS  Google Scholar 

  • Walsby AE, Armstrong RE (1979) Average thickness of the gas vesicle wall in Anabaena flos-aquae. J Mol Biol 129:279–285

    Article  CAS  Google Scholar 

  • Walsby AE, Buckland B (1969) Isolation and purification of intact gas vesicles from a blue-green alga. Nature (London) 224:716–717

    Article  Google Scholar 

  • Walsby AE, Hayes PK (1988) The minor cyanobacterial gas vesicle protein, GVPc, is attached to the outer surface of the gas vesicle. J Gen Microbiol 134:2647–2657

    CAS  Google Scholar 

  • Walsby AE, Revsbech NP, Griffel DH (1992) The gas permeability coefficient of the cyanobacterial gas vesicle wall. J Gen Microbiol 138:837–845

    CAS  Google Scholar 

  • Wong DCF, Wong KTK, Goh LT, Heng CK, Yap MGS (2004) Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures. Biotechnol Bioeng 89:164–177

    Article  CAS  Google Scholar 

  • Zeleznik MJ, Segatta JM, Ju L-K (2002) Polyethyleneimine-induced flocculation and flotation of cyanobacterium Anabaena flos-aquae for gas vesicle production. Enz Microb Technol 31:949–953

    Article  CAS  Google Scholar 

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Acknowledgements

The work was supported by a grant to Ohio Bioprocessing Research Consortium from Ohio Board of Regents and a Faculty Research Grant from The University of Akron.

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  1. Anand Sundararajan

    Present address: Martek Biosciences Corp., 555 Rolling Hills Lane, Winchester, KY, 40391, USA

Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH, 44325-3906, USA

    Anand Sundararajan & Lu-Kwang Ju

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  1. Anand Sundararajan
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  2. Lu-Kwang Ju
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Correspondence to Lu-Kwang Ju.

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Sundararajan, A., Ju, LK. Use of cyanobacterial gas vesicles as oxygen carriers in cell culture. Cytotechnology 52, 139–149 (2006). https://doi.org/10.1007/s10616-007-9044-9

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  • DOI: https://doi.org/10.1007/s10616-007-9044-9

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