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Elongated mouse chromosomes suitable for enhanced molecular cytogenetics

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Abstract

Characterization of genetic disorders in humans and animal models requires identification of chromosomal aberrations. However, identifying fine deletions or insertion in metaphase chromosomes has been always a challenge due to limitations of resolution. In this study we developed a rapid method for chromosome elongation using two different intercalating agents: ethidium bromide and 5-bromo-2′-deoxyuridine (BrdU), together with a short-term mitotic block using colcemid. About 70% of the chromosomes from cells that underwent this elongation procedure reached three times longer than those prepared from control cells. FISH experiments using elongated chromosomes revealed a duplicated region of chromosome 11 that was not visible in cells prepared with conventional methods.

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Authors and Affiliations

  1. Manitoba Institute of Cell Biology, Cancer Care Manitoba, University of Manitoba, 675 McDermot Avenue, Winnipeg, MB, R3E 0V9, Canada

    Sherif Louis, Michael Mowat & Sabine Mai

  2. Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden

    Katalin Benedek & George Klein

Authors
  1. Sherif Louis
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  2. Katalin Benedek
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  3. Michael Mowat
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  4. George Klein
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  5. Sabine Mai
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Correspondence to Sabine Mai.

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Louis, S., Benedek, K., Mowat, M. et al. Elongated mouse chromosomes suitable for enhanced molecular cytogenetics. Cytotechnology 44, 143–149 (2004). https://doi.org/10.1007/s10616-004-2978-2

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  • DOI: https://doi.org/10.1007/s10616-004-2978-2

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