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Secondary Metabolites from Alternaria sp., a Fungal Endophyte Isolated from the Seeds of Ziziphus jujuba

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Chemistry of Natural Compounds Aims and scope

From the seeds of the traditional medicinal plant Ziziphus jujuba growing in Uzbekistan, the fungal endophyte Alternaria sp. was isolated. Extracts of this fungus grown on solid rice and liquid Wickerham culture media yielded 21 natural products. These natural products include the new phthalide natural product (±)7-methoxyphthalide-3-acetic acid (1) in addition to 20 known compounds including 19 polyketides and one alkaloid. Moreover, the OSMAC (One Strain MAny Compounds) approach is applied in this study. The unambiguous structure of the new compound was elucidated on the basis of one- and two-dimensional NMR spectroscopy in addition to HR-ESI-MS. Compounds 2, 3, 4, and 7 exhibited a cytotoxic activity against mouse lymphoma cell lines (L5178Y) with IC50 values of 1.7, 7.8, 6.8, and 6.2 μM, respectively when compared with the positive control kahalalide F (IC50 4.3 μM).

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Acknowledgment

The KSU are gratefully acknowledged for (R. S. O). We are indebted to Prof. W. E. G. Muller (Johannes Gutenberg University, Mainz, Germany) for performing the cytotoxicity assay.

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Authors and Affiliations

  1. Heinrich-Heine-Universitaet, Institut fur Pharmazeutische Biologie und Biotechnologie, Universitaetsstrasse 1, Geb. 26.23, D-40225, Dusseldorf, Germany

    Raha S. Orfali & Weaam Ebrahim

  2. Department of Pharmacognosy, Faculty of Pharmacy, King Saud University, Riyadh, 2454, Saudi Arabia

    Raha S. Orfali

  3. Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt

    Weaam Ebrahim

  4. Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Sharkia, 44519, Egypt

    Azza M. El-Shafae

Authors
  1. Raha S. Orfali
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  2. Weaam Ebrahim
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  3. Azza M. El-Shafae
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Corresponding author

Correspondence to Weaam Ebrahim.

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Published in Khimiya Prirodnykh Soedinenii, No. 6, November–December, 2017, pp. 878–880.

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Orfali, R.S., Ebrahim, W. & El-Shafae, A.M. Secondary Metabolites from Alternaria sp., a Fungal Endophyte Isolated from the Seeds of Ziziphus jujuba . Chem Nat Compd 53, 1031–1034 (2017). https://doi.org/10.1007/s10600-017-2195-9

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  • DOI: https://doi.org/10.1007/s10600-017-2195-9

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